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一种基于质谱的敏感方法,用于识别儿童常见的呼吸道病原体
Lixin Hu1,2,3, Shenyan Zhang4,5, Wenqi Song3
1Capital Medical University , Beijing, China.
Microbiology spectrum
|September 27, 2023
概括
一种新的质谱检测方法可以快速检测儿童的18种常见呼吸道病原体. 这种灵敏而准确的方法为传统测试提供了具有成本效益的替代方案,改善了呼吸道感染的诊断.
科学领域:
- 临床微生物学和传染病.
- 分子诊断和质谱学分子诊断和质谱学
背景情况:
- 新出现的呼吸道感染对公共健康构成重大风险,特别是在儿科患者群体中.
- 传统的基于培养的检测方法是缓慢的,需要1-3天,延迟诊断和治疗.
- 需要为儿科呼吸道病原体提供快速,准确和经济高效的诊断工具.
研究的目的:
- 开发和评估一种新的23倍数质谱测定方法,用于同时检测儿童常见的呼吸道病原体.
- 与现有方法相比,评估这种新试验的临床性能,灵敏度,特异性和成本效益.
主要方法:
- 开发了一种23倍位的测试方法,将飞行质谱的矩阵辅助激光脱吸/离子化时间 (MALDI-TOF MS) 与多重反转录PCR (mRT-PCR) 结合起来.
- 该试验针对11种细菌和7种病毒病原体 (包括10种亚型) 和两个内部对照.
- 使用450个儿科呼吸道样本评估临床性能,将结果与实时RT-PCR进行比较.
主要成果:
- 该试验实现了低至1拷贝/μL的检测极限,没有交叉反应.
- 临床评估显示,与实时RT-PCR的一致性为96.0%,多重感染识别率为7.1%.
- 在大约7小时内实现了192个样本的同时分析,减少了样本使用和成本.
结论:
- 开发的基于MS的MALDI-TOF测定是一种敏感,准确和具有成本效益的方法,用于检测儿童常见的呼吸道病原体.
- 与传统方法相比,这种创新方法大大减少了交付时间和成本.
- 该试验有可能通过提供快速的病因诊断来彻底改变儿科呼吸道感染的诊断.
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