相关实验视频

Updated: Jul 15, 2025

The ITS2 Database

The ITS2 Database

Published on: March 12, 2012

补偿基变化在ITS2二级结构对齐,建模和分子系谱:改善Tulasnella (Basidiomycota) 的物种界限的综合方法

Yuliana Jiménez-Gaona¹, Oscar Vivanco-Galván², Darío Cruz²

¹Departamento de Química, Universidad Técnica Particular de Loja (UTPL), San Cayetano Alto s/n, Loja 1101608, Ecuador.

Journal of fungi (Basel, Switzerland)

|September 27, 2023

概括

这项研究引入了用于Tulasnella物种划界的新型分子工具,将基于对齐和无对齐的方法与二次结构分析相结合. 结果清楚地定义了七个Tulasnella物种,改善了真菌物种的识别.

科学领域:

菌类学菌类学是指菌类学.
进化生物学进化生物学
生物信息学是一种生物信息学.

背景情况:

由于形态和分子数据之间的模糊性,在真菌属*Tulasnella*中的物种划界是具有挑战性的.
结合分子,生态和形态数据的综合方法对于解决复杂的真菌物种至关重要.
之前的研究强调,需要先进的分子工具来准确地定义Tulasnella*物种.

研究的目的:

用新的分子工具评估Tulasnella*中的进化关系,包括基于对齐和无对齐的距离矩阵.
分析内部转录间隔器2 (ITS2) 序列的二次结构和保存基组成 (CBC),以改进物种划界.
为解决*Tulasnella*属内的物种界限提供一个强大的分子框架.

主要方法:

用三种方法进行了家族遗传学分析:基于对齐的,无对齐的和联合距离矩阵方法 (DISTATIS,pvclust).
ITS2序列的二级结构共识模型使用Mfold.生成.
使用4Sale进行保存基组成 (CBC) 分析,以补充物种划界.

主要成果:

遗传学分析成功地划分了单类群,将142个Tulasnella序列分为两个主要群 (A和B).
鉴定了七种不同的图拉斯内拉菌种 (*T. asymmetrica*, *T. andina*, *T. eichleriana* ECU6, *T. eichleriana* ECU4, *T. pinicola*, *T. violea*),得到了高启动值 (72-100%) 的支持.
确定了三种类型的二维二次结构共识模型,其中有九种保存基组成 (CBC) 证实了物种级别的区别.

结论:

结合的分子方法为*Tulasnella*物种划界提供了一种可靠的方法.
对ITS2序列的二次结构和CBC分析为真菌物种识别提供了有价值的补充数据.
这项研究成功地解决了Tulasnella*内的物种界限,有助于更好地了解真菌的多样性和进化.

关键词:

这就是ITS2的意义.基于对齐的对齐基于对齐.没有对齐的自由对齐.补偿基数变化是补偿基数变化.二级结构是二级结构的二次结构.种类划分种类划分

更多相关视频

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers

Published on: September 29, 2023

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

相关概念视频

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Phylogenetic Trees

Phylogenetic Trees

Phylogenetic trees come in many forms. It matters in which sequence the organisms are arranged from the bottom to the top of the tree, but the branches can rotate at their nodes without altering the information. The lines connecting individual nodes can be straight, angled, or even curved.

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

Phylogeny

Phylogeny

Phylogeny is concerned with the evolutionary diversification of organisms or groups of organisms. A group of organisms with a name is called a taxon (singular). Taxa (plural) can span different levels of the evolutionary hierarchy. For instance, the group containing all birds is a taxon (comprising the class Aves), and the group of all species of daisies (the genus Bellis) is a taxon. Phylogenies can likewise include just one genus (i.e., depict species relationships) or span an entire kingdom.

Diversity of Protists III

Diversity of Protists III

Rhizaria are a diverse group of unicellular protists characterized by their threadlike cytoplasmic extensions known as pseudopodia. These structures aid in both locomotion and feeding, giving Rhizaria an amoeboid appearance. Their amoeboid morphology once led to taxonomic confusion, but molecular phylogenetics has clarified their evolutionary placement and emphasized their shared use of pseudopodia despite divergent lineages.This clade comprises diverse lineages such as Chlorarachniophyta,...