ABCC转运基因MoABC-R1与Magnaporthe oryzae中的Pyraclostrobin耐受性有关
Pei Hu1, Yanchen Liu1, Xiaoli Zhu1
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Journal of fungi (Basel, Switzerland)
|September 27, 2023
概括
在大米爆发真菌中,一个关键基因MoABC-R1控制着杀菌剂耐受性和病原性. 删除该基因可以提高杀菌剂的有效性,为管理这种破坏性作物疾病提供了新的策略.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
背景情况:
- 由全球性真菌病引起的爆,威胁着全球粮食安全.
- 大米爆发真菌中真菌杀菌剂的耐受性损害了疾病控制工作.
- ATP结合盒 (ABC) 载体与植物病原菌中的多种药物耐受性有关.
研究的目的:
- 为了研究ABC载体在米爆真菌中Pyraclostrobin敏感性的作用.
- 为了识别与真菌杀菌剂耐受性相关的特定ABC转运基因.
- 评估已识别的基因作为疾病管理目标的潜力.
主要方法:
- 选90种大米爆发真菌菌株,以检测皮拉克洛斯特罗宾的敏感性.
- 在50个ABC转运基因中的多态性分析.
- 基因删除研究,以评估已识别的候选基因 (例如,MoABC-R1) 的功能.
- 病原性测定和真菌杀菌剂疗效评估.
主要成果:
- 鉴定了MoABC-R1,一个ABCC类型的转运基因,与pyraclostrobin耐受性相关.
- 删除突变 (abc-r1) 显示显著增加对pyraclostrobin (0.01μg/mL) 的敏感性.
- 当与杀菌剂结合使用时,MoABC-R1删除减少了真菌病原性,并将控制效率提高了三倍.
结论:
- 在米爆真菌中,MoABC-R1对于杀菌剂耐受性和致病性至关重要.
- 准MoABC-R1是一个有希望的策略,可以提高杀真菌剂的有效性和管理大米爆炸.
- 这项研究为可持续控制爆病提供了一种新方法.
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