透应激反应,细胞壁的完整性和结合是通过Trichoderma atroviride中的丁激酶传感器来调节的
Gabriela Calcáneo-Hernández1,2, Fidel Landeros-Jaime1, José Antonio Cervantes-Chávez1
1Unit for Basic and Applied Microbiology, Faculty of Natural Sciences, Autonomous University of Queretaro, Queretaro 76230, Mexico.
Journal of fungi (Basel, Switzerland)
|September 27, 2023
概括
在Trichoderma atroviride中的histidine kinase Nik1在透应激反应和细胞壁稳定性中起作用,但对于所有Tmk3通路激活来说并不必不可少. 额外的histidine激酶可能有助于应激适应.
科学领域:
- 菌类生物学 菌类生物学
- 分子生物学分子生物学
- 应激反应路径 应激反应路径
背景情况:
- 特里科德玛阿特罗维里德利用基因激活蛋白激酶 (MAPK) 途径,包括Tmk3和Pbs2,以应对环境压力因素.
- 基因酶 (HKs) 是已知的调节者Tmk3在其他真菌中,但它们在T. atroviride中的功能尚未被探索.
研究的目的:
- 为了研究 Trichoderma atroviride 中的海斯提丁激酶 Nik1 的功能.
- 确定尼克1在通过Pbs2-Tmk3信号通路调解的应激反应中的作用.
主要方法:
- 野生型 (WT) 和Dnik1突变菌株T. atroviride的比较分析.
- 评估各种压力条件下的生长,细胞壁稳定性和基因表达 (奥斯摩斯压力,溶解酶,刚果红色).
- 对光调节的基因表达 (blu1, grg2) 和奇合成酶基因 (chs1, chs2, chs3) 的分析.
主要成果:
- 德尼克1突变体在高位压力下表现出增长受损,对溶解酶的抗性降低.
- 突变 Δnik1 的状体对刚果红色更敏感,这表明细胞壁的特性发生了变化.
- 虽然Pbs2-Tmk3独立于Nik1调解了透冲击诱导的blu1和grg2表达,但Nik1影响了基因表达和光依赖的生长/结合.
结论:
- 在T. atroviride的Pbs2-Tmk3通路内,Nik1参与了特定的应激反应,特别是关于细胞壁完整性和透应激.
- 尼克1的功能与其通过Tmk3.3调节光敏感基因的作用不同.
- 这些发现表明,多个胺激酶可能会协作,以确保T. atroviride的全面应激适应.
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