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营养素缺乏对萨克西托克素产生亚历山大分钟的细胞和代谢反应的影响:一个转录的视角

Muhamad Afiq Akbar^1,2,3, Nurul Yuziana Mohd Yusof⁴, Gires Usup⁴

¹Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.

|September 27, 2023

概括

由Alexandrium minutum引起的有害藻类繁殖 (HABs) 在营养缺乏下显示出明显的细胞变化. 缺和显著改变基因表达,影响关键的代谢和信号通路在这个dinoflagellate.

科学领域:

海洋生物学海洋生物学
微生物生态学微生物生态学
毒理学毒理学毒理学

背景情况:

有害藻类繁殖 (HABs) 是一个重要的生态和经济问题.
亚历山大小是一种经常与HAB和萨克西托克辛生产相关联的恐龙鞭状体物种.
了解HAB对营养限制的生态生理反应对于预测开花动态至关重要.

研究的目的:

为了研究亚历山大微对和缺乏的转录和代谢反应.
为了确定受营养压力影响的关键细胞和分子通路,在这个HAB形成的dinoflagellate.

主要方法:

在受控和缺乏下对Alexandrium minutum培养物的转录组分析.
差异基因表达分析以识别显著改变的基因 (DEG).
基因组丰富分析 (GSEA) 用于识别丰富的基因本体学 (GO) 术语.

主要成果:

缺导致1049个差异表达基因 (DEGs) 和丰富的途径,如变,碳水化合物代谢,和离子稳态.
缺乏导致763个DEG和碳代谢,细胞离子转移和信号通路中的丰富.
具体影响包括在应力下改变氨同化和在应力下蛋白质循环.

结论:

营养素缺乏会触发亚历山大中不同的转录基因反应.
和限制差异调节关键细胞过程,影响这种HAB物种的生态生理学.
这些发现增强了我们对HABs适应不断变化的海洋环境的理解.

关键词:

基因组丰富分析分析有害的藻类的开花藻类的开花萨克西托克辛是一种萨克西托克辛.翻译学翻译学翻译学翻译学

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