在患有慢性炎症性肠道病变的狗中代谢胆汁酸概况损伤
Rossana Comito1,2, Emanuele Porru1,2, Nicolò Interino3
1Department of Medical and Surgical Sciences, Alma Mater Studiorum-University of Bologna, 40138 Bologna, Italy.
Metabolites
|September 27, 2023
概括
在患有慢性炎症性肠病症 (CIE) 的狗中,便胆酸概况显著不同. 特定的氧胆酸 (oxo-BAs) 与CIE的可能性较低有关,这表明它们的诊断潜力.
科学领域:
- 胃肠病学 胃肠病学
- 代谢学 代谢学 代谢学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 胆汁酸 (BAs) 是肝脏中合成的关键内源性类固醇,在肝毒性和炎症性肠病等疾病中影响肠肝轴.
- 便氧胆酸 (oxo-BAs),BA代谢的稳定中间体,提供了关于肝肠交叉声和与疾病相关的微生物转移的见解.
- 有限的数据存在于狗便的BA档案,特别是在患有慢性炎症性肠病 (CIE) 的狗中.
研究的目的:
- 综合评估和比较慢性炎症性肠病 (CIE) 的狗和没有狗的便胆酸概况.
- 研究特定便胆酸 (包括oxo-BAs) 与CIE存在或不存在之间的关联.
- 探索与CIE和没有CIE的狗中微生物群相关代谢物的差异.
主要方法:
- 使用高性能液态染色体双重质谱法 (HPLC-MS/MS) 对31种便胆酸和相关代谢物的综合分析.
- 使用后勤回归分析来估计BA和CIE之间的关联的几率比率 (OR).
- 进行了主要成分分析 (PCA),以确定对照和病态犬群之间的不同代谢配置文件.
主要成果:
- 患有CIE的狗表现出明显较高的初级胆酸和木里可酸水平,以及较低的二级胆酸水平.
- 便中氧代谢物度升高与患有CIE的几率降低有关 (OR < 1).
- 在有或没有CIE的狗之间观察到明显的便胆酸概况.
结论:
- 在CIE和没有CIE的狗之间,便胆酸概况存在明显的差异.
- 在没有CIE的情况下,便中的oxo-BAs可能起着保护作用或表明肠道环境更健康.
- 需要进一步调查oxo-BAs和muricholic酸在狗CIE中的作用.
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