针对氨基酸-OMICS的向GC-MS的质量控制
Dimitrios Tsikas1, Bibiana Beckmann1
1Core Unit Proteomics, Institute of Toxicology, Hannover Medical School, 30623 Hannover, Germany.
Metabolites
|September 27, 2023
概括
这项研究引入了一种新的质量控制系统,用于使用气色谱质谱法 (GC-MS) 分析人体血中的氨基酸. 该系统确保了氨基酸和代谢物的准确量化,提高了OMICS数据的可靠性.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 代谢学 代谢学 代谢学
背景情况:
- 气色谱-质谱 (GC-MS) 是分析非极性化合物的强大工具.
- 自由氨基酸 (AA) 是极性,非挥发性和热不稳定的,需要用于GC-MS分析的衍生.
- 对像血这样的生物样本中氨基酸的准确量化对于OMICS研究至关重要.
研究的目的:
- 开发和验证一种新的OMICS兼容的质量控制 (QC) 系统,用于人类血中自由氨基酸的定量GC-MS分析.
- 评估QC系统对分析21种自由氨基酸和代谢物的有用性.
- 评估基于保留时间的同位素效应和保留时间差异作为定性分析的参数.
主要方法:
- 氨基酸被衍生为它们的甲基 (Me) 和随后的五烯 (PFP) 衍生物.
- 脱甲基 (d3Me-AA-PFP) 在现场合成,并作为内部标准使用.
- 实施了一个质量控制系统,涉及水溶液和汇集的人类血样本的交叉标准化.
主要成果:
- 开发的质量控制系统证明了对人体血中21种自由氨基酸和代谢物的定量分析的有效质量控制.
- 基于保留时间的同位素效应 (IE) 和保留时间差异 (δ(H/D)) 确定了d0Me-AA-PFP和d3Me-AA-PFP衍生物.
- 在七个工作日内,在八个运行中分析了400多个血样本,表明了高吞吐量和可重复性.
结论:
- 拟议的质量控制系统提供了关于GC-MS分析人体血中氨基酸的定量性能的可靠信息.
- 同位素效应,保留时间差异和化内部标准的峰值面积下降可以作为有价值的参数用于针对性GC-MS氨基酸-OMICS的定性分析.
- 这种方法可以在OMICS研究中对生物氨基酸进行高通量量化测量.
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