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Updated: Jul 15, 2025

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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在初级海马神经元中基于CRISPR的可编程RNA编辑.

Karthick Ravichandran1, Tulika Khargonkar1, Sarbani Samaddar1

  • 1National Brain Research Centre, Manesar, Haryana, India.

Current protocols
|September 27, 2023
PubMed
概括

研究人员调整了基于CRISPR的RNA编辑工具,以可视化神经元中的RNA编辑. 这种新方法可以在不改变基因组的情况下精确操纵RNA功能,为研究RNA-蛋白相互作用铺平了道路.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?在Cas13b中使用.丹德拉2 的意思编辑RNA的RNA可以被编辑.这就是 crRNARNA.在小鼠中,初级海马体培养.

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