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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Formation of Muscle Fibers from Myoblasts01:13

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De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
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Induced Pluripotent Stem Cells01:06

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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相关实验视频

Updated: Jul 15, 2025

Chromatin Isolation by RNA Purification ChIRP
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Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

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作为"MYC促进者"的长非编码RNA.

Daniel García-Caballero1, Jonathan R Hart1, Peter K Vogt1

  • 1Department of Molecular Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Pathophysiology : the official journal of the International Society for Pathophysiology
|September 27, 2023
PubMed
概括
此摘要是机器生成的。

研究人员确定了"MYC促进者",这是一类长非编码RNA (lncRNAs),它们支持MYC驱动的细胞增殖,但对细胞存活至关重要. 这些与MYC相互作用的lncRNA是了解MYC的关键.

关键词:
克里斯普里是指克里斯普里.克里斯普尔卡斯9在 lncRNAs 中.转录因子RNA复合体转录规则 转录规则 转录规则

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科学领域:

  • 分子生物学分子生物学
  • 癌症研究 癌症研究
  • 遗传学 遗传学 是一个

背景情况:

  • MYC瘤基因在细胞增殖,生存和新陈代谢中起着至关重要的作用.
  • 长非编码RNAs (lncRNAs) 越来越被认为是基因表达和细胞过程的关键调节者.
  • MYC通过 lncRNAs 影响细胞功能的精确机制尚未完全理解.

研究的目的:

  • 定义和描述一类由MYC直接调节的特定类 lncRNAs.
  • 研究这些MYC相互作用的lncRNAs在MYC驱动的细胞过程中的功能作用.
  • 提出关于lncRNAs参与MYC介导的转录调节的假设.

主要方法:

  • 基于以下标准确定lncRNAs:MYC的直接转录标,与MYC协调表达,对持续的MYC驱动的增殖的要求,以及对细胞存活的非必要性.
  • 详细分析了两个代表性的lncRNAs:SNHG17 (小核RNA宿主基因17) 和LNROP (POU2F2的长非编码调节器2).
  • 关于lncRNAs在MYC的转录调节网络中的作用的一般假设的制定.

主要成果:

  • 确定了一种不同的lncRNA类,称为"MYC促进者".
  • 这些MYC促进者是MYC的直接转录目标,它们的表达与MYC水平协调.
  • SNHG17和LNROP被介绍为MYC促进者的关键例子,对于持续扩散至关重要,但不是生存.

结论:

  • 在维持MYC驱动的细胞增殖中,MYC促进体起着至关重要的作用.
  • 这些lncRNAs在MYC介导的细胞过程中代表了一种新的调节层.
  • 对MYC促进者的进一步研究可能会在MYC驱动的癌症中揭示新的治疗点.