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Updated: Jul 15, 2025

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

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用dHis-Cu2+ (NTA) 基因在脉冲双极EPR光谱学中区分标签和蛋白质符合者

Caspar A Heubach1, Zikri Hasanbasri2, Dinar Abdullin1

  • 1Clausius-Institute of Physical and Theoretical Chemistry, University of Bonn, Wegelerstr. 12, 53115, Bonn, Germany.

Chemistry (Weinheim an der Bergstrasse, Germany)
|September 27, 2023
PubMed
概括

一个硬的旋转标签,铜II-酸结合两个histidines (dHis-Cu2+NTA),揭示了Yersinia外蛋白O (YopO) 的单个构造,使用脉冲双极EPR光谱 (PDS). 这种方法简化了结构生物学分析.

关键词:
铜铜 铜铜的铜.双重的西斯蒂丁是没有的.电子偏磁共振光谱学 电子偏磁共振光谱学脉冲电子-电子双共振光谱学 (PELDOR) 是一种技术.放松诱导的双极调制增强光谱学 (RIDME)

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