在小羊养小麦,多年生小麦或多年生小麦加上白的羊皮表皮蛋白质的定量差异
Jude Jessie Bond1, Gordon Refshauge2, Matthew T Newell2
1NSW Department of Primary Industries, Extensive Livestock Industry Centre, Trevenna Rd, University of New England, Armidale, NSW 2351, Australia.
Proteomes
|September 27, 2023
概括
与常规小麦 (W) 相比,多年生小麦 (PW) 和花 (PWL) 饮食没有改变皮表皮的营养载体. 然而,PWL饮食显著改变了皮上皮细胞中脂肪酸和能量代谢途径中的蛋白质丰度.
科学领域:
- 动物科学动物科学
- 类动物营养 类动物营养
- 蛋白质组学是指蛋白质组学.
背景情况:
- 多年生小麦 (PW) 的营养价值及其与 (PWL) 结合用于畜牧放牧和粮食生产需要进一步调查.
- 了解不同料饮食对皮上皮 (RE) 功能的影响,对于优化动物健康和生产力至关重要.
研究的目的:
- 研究常规小麦 (W),多年生小麦 (PW) 和多年生小麦-白 (PWL) 饮食对参与羊母上皮质中营养运输的膜结合蛋白的影响.
- 为了确定受这些饮食影响的特定代谢途径,在皮表皮中的蛋白质水平上.
主要方法:
- 杂交羊被新鲜切割的W,PW或PWL (50:50) 料食四周.
- 蛋白质组分析被用来比较不同饮食群体的皮表皮中的蛋白质丰富度.
- 统计分析,包括错误发现率 (FDR) 和本杰明尼-霍赫伯格校正,用于识别显著的蛋白质变化.
主要成果:
- 与养PW或PWL饮食的羊相比,养常规小麦 (W) 的羊羔的平均每日益 (ADG) 和代谢能量摄入量 (MEI) 显著更高.
- 在RE中,没有观察到与营养物质运输或能量代谢相关的蛋白质丰度在W与PW和W与PWL养的羔羊之间有显著的差异.
- 在PW和PWL饮食之间对对进行的比较显示,脂肪酸代谢,氧化酸化和辅因子生物合成的途径内的蛋白质丰度存在显著差异,特别是影响线粒体蛋白质.
结论:
- 传统的小麦饮食支持较高的生长率和能量摄入,与多年生小麦的饮食相比,羊羔.
- 虽然多年生小麦和花的含有没有影响皮表皮中的营养物质运输蛋白质,但它们显著改变了参与脂肪酸和能量代谢的蛋白质,这表明细胞代谢功能发生了转变.
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