在组织样本上测试不同的消化溶液,以及使用的氧化溶液对聚烯微球的影响
Liesa Geppner1, Jakob Karaca1, Wencke Wegner2
1Department of Medicine, Faculty of Medicine and Dentistry, Danube Private University, Steiner Landstraße 124, 3500 Krems, Austria.
Toxics
|September 27, 2023
概括
研究人员开发了一种低成本,非破坏性的方法,使用氧化 (KOH) 来从器官组织中分离微塑料. 这种方法有效地溶解各种样品,保持微塑料的完整性,以便在食物链中检测.
科学领域:
- 环境科学 环境科学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 微塑料颗粒污染了各种环境,包括空气,水,土壤和食物链.
- 微塑料的小尺寸引发了人们对其进入血液和在器官中积累的可能性的担忧.
- 精确检测微塑料需要有效的隔离技术从生物矩阵.
研究的目的:
- 开发一种非破坏性,高效和负担得起的方法,以从器官组织中分离微塑料颗粒.
- 评估不同消化方法的有效性,包括酶和化学处理,用于样品制备.
- 评估选择的隔离方法对常见微塑料类型的完整性的影响.
主要方法:
- 使用蛋白酶K,素/胰腺素和10%的氧化 (KOH) 溶液,消化猪,肺,肝和大脑样本.
- 通过光显微镜分析脏样本.
- 优化KOH:组织比率用于肺部,肝脏和大脑样本的消化.
- 扫描电子显微镜检查10%的KOH对聚乙烯微塑料的影响.
主要成果:
- 10%的氧化 (KOH) 溶液被证明是最有效的溶解各种器官样本.
- 酶性消化方法需要进一步优化微塑料的隔离.
- 在10%的KOH溶液中化76小时后,1μm的聚乙烯微塑料保持完整.
- KOH消化是一种简单,经济高效的器官样本处理方法.
结论:
- 氧化 (KOH) 消化为从器官组织中非破坏性分离微塑料提供了一个有希望的方法.
- 这种方法在分析肉制品中的微塑料污染方面具有潜在的应用.
- 对于特定的应用,可能需要进一步改进酶方法.
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