研究形态变化和干扰艾滋病的发展,由一些必需油成分引起的研究
Michele Teixeira Serdeiro1,2, Thiago Dutra Dias2,3, Natanael Teles Ramos de Lima4
1Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil.
Tropical medicine and infectious disease
|September 27, 2023
概括
尼罗利多尔和法内索尔等精油显示出强大的杀性活性对抗埃及蚊虫,为控制登革热,奇孔古尼亚和寨卡病毒爆发提供了一种自然方法.
科学领域:
- 医学昆虫学 医学昆虫学
- 自然产品化学 自然产品化学
- 毒理学 毒理学 毒理学
背景情况:
- 登革热, Chikungunya 和 Zika 是由 Aedes aegypti 蚊子传播的树状病毒,对全球健康构成重大风险.
- 有效控制AE的有效控制. 埃吉普蒂种群对于防止树冠病毒爆发至关重要.
- 精油成分因其生物活性而得到认可,包括潜在的杀性质.
研究的目的:
- 为了评估六种精油成分的幼虫杀虫效果:二石,p-cymene,carvacrol,thymol,farnesol和 nerolidol.
- 为了研究这些化合物对Aedes aegypti幼虫的形态的影响.
- 确定最有效的天然化合物来控制埃及蚊子.
主要方法:
- 第三个阶段的幼虫 Ae. 埃吉普蒂被暴露在不同度 (1-100微克/毫升) 的精油成分溶于二甲基硫氧化物中.
- 评估了杀性活性,并确定了致死度50 (LC50) 的值.
- 在样本准备,脱水,嵌入和对比后,使用传输电子显微镜检查治疗幼虫的形态变化.
主要成果:
- 尼罗利多尔表现出最高的幼虫杀伤效率,LC50为11μg/mL,其次是法内索尔 (LC50: 21μg/mL),p-cymene (LC50: 23μg/mL),卡瓦克罗尔 (LC50: 40μg/mL),蒂摩尔 (LC50: 45μg/mL) 和二水素 (LC50: 66μg/mL).
- 用这些精油成分治疗诱导了幼虫体和内部结构的形态变化.
- 所有测试的化合物都显示出对AE的有效杀活性. 埃及幼虫. 埃及幼虫.
结论:
- 尼罗利多尔,法尔内索尔,p-cymene,卡瓦克罗尔,蒂摩尔和二水素具有显著的杀性质,可以对抗Aedes aegypti.
- 这些天然化合物为开发环保策略来控制蚊子载体提供了有希望的途径.
- 这些发现支持使用精油作为一种可持续的方法来缓解树冠病毒传播.
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