作为蛇毒杀伤性中和测试的人性终点的体温下降
Rosa De Jesus1, Adam E Tratner2,3, Alanna Madrid1
1Bioterio, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), City of Knowledge, Panama City 0843-01103, Panama.
Toxins
|September 27, 2023
概括
蛇毒抗毒试验导致动物的痛苦. 测量小鼠的体温可以预测生存率,潜在地减少动物的痛苦和提高测试准确度.
科学领域:
- 毒理学 毒理学 毒理学
- 动物科学动物科学
- 生物技术是生物技术.
背景情况:
- 蛇毒中和功效测试对于抗毒品质量控制至关重要.
- 目前使用小鼠的方法会给动物带来很大的痛苦和痛苦.
- 预测动物死亡的临床标志观察是主观的,可能会影响结果.
研究的目的:
- 评估体温测量作为一个客观参数,用于预测蛇毒试验中的动物生存率.
- 探索在抗毒药效力测试中建立一个人道的终点的潜力.
- 为了减少蛇毒中和功效评估期间动物的痛苦.
主要方法:
- 给BALB/c小鼠注射了 *Bothrops asper* 或 *Lachesis stenophrys* 毒素和多价抗毒素的混合物.
- 红外温度计被用来测量小鼠体温的变化.
- 分析了从基线下降的温度,以预测动物的生存率.
主要成果:
- 在注射后的前3小时内,观察到温度下降和动物生存之间的显著相关性.
- 身体温度的变化提供了一个客观的衡量标准来预测毒素暴露的结果.
- 这种方法为主观临床征兆评估提供了潜在的替代方案.
结论:
- 测量体温可以作为一个客观的指标,预测蛇毒毒力测试的生存率.
- 实施温度监测可能会导致更准确和更人道的终点,减少动物的痛苦.
- 这种方法符合在生物质量控制中尽量减少动物使用和痛苦的伦理趋势.
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