在意大利谷物中进行多种真菌毒素分析,使用超高性能染色学与四极轨道轨道质谱相结合
Juliane Lima da Silva1, Sonia Lombardi2, Luigi Castaldo2
1School of Chemistry and Food, Federal University of Rio Grande, Av. Itália, Km 8, Rio Grande 96203-900, RS, Brazil.
Toxins
|September 27, 2023
概括
这项研究开发了一种快速检测意大利谷物谷物中的21种真菌毒素的方法. 发现了11种真菌毒素,其中alpha-zearalenol是最常见的,而deoxynivalenol的水平最高,这突显了食品安全方面的担忧.
科学领域:
- 食品化学 食品化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 谷物中的真菌毒素对人类健康构成重大风险,并造成经济损失.
- 确保食品的安全和质量是一个日益关注的问题.
研究的目的:
- 开发和验证一个全面的分析策略,用于检测谷物谷物中的21种真菌毒素.
- 评估意大利谷物样本中真菌毒素的发生和同时发生情况.
主要方法:
- 一种简单,快速的样品制备方法与超高性能液态色谱学相结合,与四极轨道高分辨率质谱学 (UHPLC Q-Orbitrap HRMS) 相结合.
- 200个意大利谷物谷物样本的分析.
主要成果:
- 该方法成功检测了21种向菌毒素中的11种.
- 阿尔法-zearalenol是最经常检测到的真菌毒素,而脱氧尼瓦伦醇显示了最高的污染水平.
- 在15.5%的样本中观察到毒素的同时出现,同时发现多达三种毒素.
结论:
- 开发的UHPLC Q-Orbitrap HRMS方法对复杂矩阵中的多菌毒素分析具有敏感性和效率.
- 调查结果提供了关于谷物中受监管和新出现的真菌毒素的发生和同时发生的有价值的数据.
- 这项研究有助于保障食品质量和安全,特别是在未受监管的真菌毒素方面.
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