对比传统和以毒素为导向的方法,用于制造抗毒剂,对抗Bitis arietans蛇毒
Felipe Raimondi Guidolin1, Kemily Stephanie de Godoi1, Angela Alice Amadeu Megale1
1Immunochemistry Laboratory, Butantan Institute, São Paulo 05503-900, Brazil.
Toxins
|September 27, 2023
概括
使用原始毒素或特定毒素为拉塞尔蛇 (Bitis arietans) 制造抗毒素显示出局限性. 研究人员提出了一种可定制抗毒剂的新模型,用于打击撒哈拉以南非洲的蛇咬死亡.
科学领域:
- 生物化学 生化学
- 免疫学 免疫学 免疫学
- 毒理学 毒理学 毒理学
背景情况:
- 蛇咬毒在撒哈拉以南非洲是一个重要的公共卫生问题,而蛇 (Bitis arietans) 是死亡的主要原因.
- 比蒂斯·阿里坦斯 (Bitis arietans) 的毒素是一种复杂的毒素混合物,包括金属蛋白酶,血清蛋白酶,脂酶,讲素和分解素.
研究的目的:
- 为了比较两种抗毒生产方法的疗效:传统的原毒免疫与使用特定毒素成分的以毒素为导向的方法.
- 为了评估免疫反应,毒素中和能力,并通过这些方法产生的抗毒素的交叉反应.
主要方法:
- 比蒂斯·阿里坦斯 (Bitis arietans) 的毒液使用尺寸排除色谱进行了分离.
- 在BALB/c小鼠中,使用原始毒素,血清蛋白酶或金属蛋白酶进行免疫.
- 评估了等离子体识别特征,抗体标位,亲和力和体外酶抑制.
- 在体内进行了保护研究.
主要成果:
- 与抗金属蛋白质酶等离子体相比,抗Bitis arietans和抗血清蛋白质酶等离子体表现出类似的识别配置文件,比抗金属蛋白质酶等离子体更高的标位和亲和力.
- 观察到其他Bitis毒物的交叉识别,但强度较低.
- 虽然在体外实现了毒素酶活性的抑制,但在体内无法实现保护.
结论:
- 传统的原始毒药和以毒素为导向的方法都在生产有效的抗毒药时表现出局限性.
- 开发可定制的多克隆,特定物种,单价抗毒的拟议模型可以作为撒哈拉以南非洲多价血清的基础.
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