相关实验视频
Updated: Jul 15, 2025

07:43
Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
20.0K
在胃活检和血清中通过PCR和ELISA检测Helicobacter suis感染的协议
Hidenori Matsui1, Masato Suzuki2, Sae Aoki3
1Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo 208-0011, Japan; Department of Bacteriology, Nagoya University Graduate School of Medicine, Aichi 466-8550, Japan; Ōmura Satoshi Memorial Institute, Kitasato University, Tokyo 108-8641, Japan.
STAR protocols
|September 27, 2023
概括
这项研究引入了一种可靠的检测Helicobacter suis感染的协议,Helicobacter suis感染是胃病的常见原因. 新方法使用PCR,细菌培养,全基因组测序和ELISA进行准确的诊断.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 胃肠病学 胃肠病学
背景情况:
- 感染Helicobacter suis是一种导致胃病的重要原因.
- 目前对H. suis的诊断方法不可靠,导致诊断不足.
- 准确的检测对于有效的疾病管理和治疗至关重要.
研究的目的:
- 为可靠检测Helicobacter suis感染而制定和提出一个全面的协议.
- 建立采样,分子检测和血清学评估的标准化程序.
- 为了提高与H. suis相关的胃病的诊断准确度.
主要方法:
- 收集胃活检和患者血清.
- DNA提取和PCR针对H. suis特定基因.
- 在一次性和二次性亚格板上培养细菌.
- 孤立细菌的全基因组测序.
- 与酶相关的免疫吸收试验 (ELISA) 用于检测血清抗体.
主要成果:
- 本方案允许在胃样本中检测H. suis.
- 分子,培养,测序和血清学方法的整合提供了一个强大的诊断方法.
- 该协议概述了检测过程的每个阶段的详细步骤.
结论:
- 开发的协议为诊断Helicobacter suis感染提供了一种可靠的方法.
- 这种标准化的方法可以改善与H. suis相关的胃病的识别.
- 进一步应用该协议可以帮助流行病学研究和临床管理.
相关概念视频
Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies
154
Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
154
Serum Laboratory Studies, Stool Test, Breath Test
368
Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
368

