关于具有皮下B16F1瘤和单细胞分析的PPP1R15A抑制小鼠模型机构的协议
Rongjing Wang1, Minghui Wang2, Siyu Pei3
1Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai, China.
STAR protocols
|September 27, 2023
概括
这项研究详细介绍了一项协议,以调查PPPP1R15A抑制剂Sephin1如何影响小鼠黑色素瘤模型中的抗瘤免疫力. 该方法整合了单细胞转录组和TCR测序,用于全面分析.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- PPP1R15A抑制剂Sephin1正在研究其在癌症治疗中的潜力.
- 了解它对抗瘤免疫力的影响对于治疗开发至关重要.
- B16F1黑色素瘤是研究小鼠皮下瘤的常见模型.
研究的目的:
- 提出研究Sephin1对抗瘤免疫力影响的详细方案.
- 建立整合单细胞转录组和T细胞受体 (TCR) 测序数据的方法.
- 为了在B16F1皮下瘤模型中对免疫反应进行全面分析.
主要方法:
- 为单细胞转录组和TCR库的构建和测序开发协议.
- 用于整合表达和TCR数据的生物信息管道.
- 对基因分化,调节子活动和细胞间通信的分析.
- 单细胞分析结果的验证.
主要成果:
- 该协议允许详细探索Sephin1对抗瘤免疫力的影响.
- 转录基因和TCR数据的整合提供了对免疫反应的多方面的观点.
- 分析揭示了对基因表达,调节网络和细胞间相互作用的见解.
- 该方法允许对观察到的免疫学效应进行可靠的验证.
结论:
- 该协议为研究Sephin1.1的免疫调节作用提供了一个框架.
- 综合单细胞方法有助于更深入地了解瘤免疫力.
- 该方法适用于其他抑制剂和瘤模型,以推进癌症免疫治疗研究.
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