从马来西亚半岛的休湖中分离的Acanthamoeba的分子表征和潜在致病性分析
Rohaya Abdul Halim1, Rosnani Hanim Mohd Hussain2, Shafiq Aazmi3
1Centre for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, Puncak Alam, Selangor, Malaysia
Journal of water and health
|September 27, 2023
概括
在马来西亚的休湖泊中发现了潜在的公共卫生问题Acanthamoeba. 基因型T4是最常见的,有几种基因型表现出高致病性,表明对公共健康的风险.
科学领域:
- 环境微生物学 环境微生物学
- 公共卫生 公共卫生
- 寄生虫学的寄生虫学
背景情况:
- 阿坎塔梅巴是一种机会主义的原生虫寄生虫.
- 娱乐用水体可以作为Acanthamoeba的水库.
- 了解Acanthamoeba的基因型和病原性对于公共卫生风险评估至关重要.
研究的目的:
- 在马来西亚的娱乐湖泊中识别Acanthamoeba基因型.
- 评估已识别的Acanthamoeba基因型的致病潜力.
- 为了将水质参数与Acanthamoeba的存在相关联.
主要方法:
- 在马来西亚的三个娱乐湖泊取水样.
- 物理化学水质分析.
- 隔离Acanthamoeba,提取DNA,并进行PCR识别.
- 基因型和生理耐受性测试.
主要成果:
- 在30个水样中的26个 (86.7%) 检测到Acanthamoeba.
- 基因型T4是最常见的,在比鲁湖 (100%),蒂蒂旺萨湖 (80%) 和沙阿勒姆湖 (80%) 发现.
- 在Acanthamoeba存在和ORP,温度,pH和DO之间观察到显著的相关性. 八个分离物 (30.8%) 呈现出高致病性.
结论:
- 马来西亚的休湖泊是Acanthamoeba的港湾,其中基因型T4占主导地位.
- 病原性阿坎塔梅巴基因型的存在构成了潜在的公共卫生风险.
- 水质参数影响Acanthamoeba的分布,需要进一步监测.
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