通过低强度固定频率连续超声波增强热友性Bacillus licheniformis YYC4的生长
Chunhua Dai1, Zhenzhen Shu2, Xueting Xu2
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China.
Ultrasonics sonochemistry
|September 27, 2023
概括
低强度固频连续超声波 (LIFFCU) 通过增强细胞透性和代谢活性,显著增强Bacillus licheniformis YYC4生物量. 这种方法提供了一种有效的方式来促进有针对性的微生物生长.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 声学生物学 声学生物学
背景情况:
- 细菌菌YYC4是一种具有工业应用的微生物.
- 优化微生物生长对于各种生物技术过程至关重要.
- 了解超声波等物理刺激对微生物生理学的影响至关重要.
研究的目的:
- 研究低强度固频连续超声波 (LIFFCU) 对Bacillus licheniformis YYC4.4.的生长和生理学的影响.
- 探索LIFFCU影响微生物生长的潜在机制,包括形态和活动变化.
- 确定LIFFCU作为一种促进向微生物生长的方法的潜力.
主要方法:
- 用28kHz,1.5h和120W (功率密度为40W/L) 的LIFFCU治疗YYC4菌株.
- 使用定量测量对生物质变化的分析.
- 扫描电子显微镜 (SEM) 来观察形态变化和声波穿孔.
- 细胞膜潜力的测量,电导率,细胞外蛋白质和核酸含量,细胞内Ca2+的测量.
- 检测细胞外蛋白酶活性,挥发性成分,微生物代谢活性和子发芽.
主要成果:
- 在LIFFCU治疗后的对数转移阶段观察到B. licheniformis YYC4生物量显著增加48.95%.
- SEM揭示了sonoporation,导致膜的透性增加,由细胞膜潜力,导电性和细胞外/细胞内物质度的升高证明.
- LIFFCU治疗显著增强了细胞外蛋白酶活性,挥发性成分,微生物代谢活性和子发芽.
结论:
- LIFFCU有效地促进了Bacillus licheniformis YYC4.4.的生长.
- 该机制涉及超声波诱导的声波穿孔,增加膜的透性,促进营养吸收和代谢交换.
- 在生物技术应用中,LIFFCU提出了一种有希望的,有效的战略,用于增强生物技术应用中特定微生物菌株的生长.
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