鱼Rhbdd3通过RIG-I信号通路积极调节IFN反应
Ling Shao1, Minghui Zhang1, Yanan Liu1
1Shanghai Fisheries Research Institute, Shanghai Fisheries Technical Extension Station, Shanghai, 200433, China.
Fish & shellfish immunology
|September 27, 2023
概括
在普通鱼中含有形域的蛋白3 (Rhbdd3) 通过调节RIG-I信号通路并抑制鱼病毒 (SVCV) 复制的春季病毒性病变来增强抗病毒免疫力. 它的C端对这种抗病毒活性至关重要.
科学领域:
- 水生免疫学 水生免疫学
- 天生的抗病毒免疫力.
- 分子病毒学分子病毒学.
背景情况:
- 含有形域的蛋白3 (Rhbdd3) 调节哺乳动物的先天免疫力.
- 鱼类Rhbdd3在病毒感染反应中的作用在很大程度上是未知的.
研究的目的:
- 描述病毒感染期间来自常见鱼 (CcRhbdd3) 的Rhbdd3的功能和调节机制.
- 调查CcRhbdd3对鱼病毒 (SVCV) 春季病毒病的抗病毒活性.
主要方法:
- 克隆和CcRhbdd3.3.的遗传学分析.
- 在感染SVCV的普通鱼体内体表分析.
- 在敏感细胞中进行过度表达和淘汰实验.
- RNA测序 (RNA-seq) 用于识别受调节的基因.
- 亚细胞局部化和截断试验.
主要成果:
- 在多个鱼组织中,SVCV感染调高了CcRhbdd3的表达.
- 过度表达CcRhbdd3抑制了SVCV的传播,而敲击则促进了病毒复制.
- CcRhbdd3过度表达上调了关键的RIG-I信号通路基因和NF-κB.
- CcRhbdd3定位在细胞质和早期内体,其C端对抗病毒功能至关重要.
结论:
- CcRhbdd3在常见鱼对SVCV的天生的免疫反应中发挥着重要作用.
- CcRhbdd3激活RIG-I信号和NF-κB通路,从而提供抗病毒保护.
- CcRhbdd3的C端对其局部和抗病毒活性至关重要,为对抗SVCV的治疗策略提供了潜在的潜力.
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