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在金色庞巴诺,Trachinotus ovatus的viperin的分子和功能特征

Lin Huang¹, Xiaowen Zhu², Jihui Kuang³

¹Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR China.

Fish & shellfish immunology

|September 27, 2023

概括

金色的庞巴诺毒蛇 (To-viperin) 被克隆,并研究了它的抗病毒作用. 托维佩林通过减少病毒复制和提高鱼类免疫基因的调节来增强抗病毒免疫力.

科学领域:

水产养殖是水产养殖的一种方式.
免疫学免疫学免疫学
病毒学病毒学

背景情况:

激进的S-adenosyl metionin域含有蛋白2 (RSAD2),或viperin,对于抗病毒免疫是至关重要的.
黄金 (Trachinotus ovatus) 中毒毒素的功能仍然未被探索.

研究的目的:

为了克隆和表征一个viperin同类物 (To-viperin) 从金色的pompano.
为了研究To-viperin在对群虹膜病毒 (SGIV) 和神经亡病毒 (NNV) 感染的反应中的作用.

主要方法:

克隆和对To-viperin基因的序列分析.
基因表达分析作为对病毒挑战和多I:C治疗的反应.
在体外使用To-viperin过度表达的抗病毒试验.

主要成果:

托-维佩林与其他鱼类维佩林同类物共享保存域.
托-维佩林表达是构成性的,由病毒感染 (SGIV,NNV) 和多I:C引起的.
过度表达的To-viperin显著降低了SGIV和NNV标位,并提高了抗病毒基因的调节 (IFNc,IRF3,ISG15).

结论:

金色庞巴诺毒蛇素 (To-viperin) 在抗病毒防御中起着重要作用.
维佩林的抗病毒功能似乎在鱼类物种中得到保护,类似于其他脊椎动物.

关键词:

免疫反应的免疫反应.它们中的一个是Trachinotus ovatus.维佩林是维佩林的一种.病毒感染病毒感染.

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