可以通过IRAK1/TAK1/IKK通路缓解诱导的CIK细胞烧亡和炎症
Xiunan Lv1, Mengyao Ren1, Tong Xu1
1College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China.
Fish & shellfish immunology
|September 27, 2023
概括
(Se) 通过减少氧化应激和炎症,保护鱼类细胞免受 (Pb) 毒性影响. 这种必不可少的微量元素减轻了由暴露引起的细胞烧灭和免疫功能障碍.
科学领域:
- 环境毒理学环境毒理学
- 水生动物卫生 水生动物卫生
- 细胞生物学 细胞生物学
背景情况:
- (Pb) 是一种有毒的重金属污染物,影响水生生物.
- (Se) 是一种重要的微量元素,具有抗氧化和抗炎性质.
- 对鱼细胞中的细胞性热和免疫功能的影响,以及的保护作用,需要进一步研究.
研究的目的:
- 调查暴露对草 (CIK) 细胞中细胞性热和免疫反应的影响.
- 确定对CIK细胞中诱导毒性的保护作用.
- 阐明对抗毒性的潜在机制.
主要方法:
- 草的细胞暴露于 (Pb) 和 (Se).
- 评估了氧化应激标志物,包括活性氧物种 (ROS) 水平.
- 分析了关键的热致死标记物 (GSDMD,NLRP3) 和炎症性细胞因子 (IL-1β,IL-18) 的表达.
- 研究了IRAK1/TAK1/IKK信号通路的激活.
主要成果:
- 暴露诱导了CIK细胞中的氧化应激,热和炎症.
- 暴露于Pb激活了IRAK1/TAK1/IKK通路,并上调了烧亡标记物 (GSDMD,NLRP3) 和炎症细胞因子 (IL-1β,IL-18).
- 治疗显著降低了ROS水平,烧标记物和炎症性细胞因子表达,减轻了Pb诱导的细胞损伤.
结论:
- 可以缓解诱导的细胞热和鱼细胞中的免疫功能障碍.
- 通过减少氧化应激和抑制IRAK1/TAK1/IKK通路来发挥其保护作用.
- 这项研究强调了在减轻水产养殖中重金属毒性的潜力.
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