一个系统的分析海洋溶解素和前病毒
Yi Yi1, Shunzhang Liu1, Yali Hao1,2
1State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Development Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Nature communications
|September 27, 2023
概括
海洋病毒及其宿主细菌广泛存在,超过40%的海洋 prokaryotic 基因组含有 proviruses. 这些 lysogens 和病毒显著影响海洋生态系统,特别是在深海.
科学领域:
- 海洋微生物学 海洋微生物学
- 病毒学 病毒学
- 基因组学就是基因组学.
- 生态生态学 生态生态学
背景情况:
- 病毒在海洋环境中丰富多样.
- Prokaryotes 形成了海洋食物网的基础.
- 了解病毒 - 核细胞相互作用对于海洋生态系统动态至关重要.
研究的目的:
- 系统地分析海洋 prokaryotic 和病毒基因组数据.
- 在海洋生态系统中识别和描述 lysogens 和 proviruses.
- 研究海洋病毒对其宿主的生态作用和影响.
主要方法:
- 海洋原生物基因组数据集 (MPGD) 和海洋温带病毒基因组数据集 (MTVGD) 的汇编.
- 对基因组序列的生物信息分析,以确定前病毒序列和病毒基因.
- 对 lysogens 和非 lysogens 的比较基因组分析.
- 使用海洋细菌的转录基因分析进行实验验证.
主要成果:
- 在MPGD中,超过40%的海洋 prokaryotic 基因组是 lysogens,含有 proviral 序列.
- 该MTVGD包括超过12,900种病毒结合体,分为10,897种病毒属.
- Lysogens 和 proviruses 在海洋生态系统中普遍存在,特别是在深海.
- 与非溶解素相比,海洋溶解素表现出明显的基因组特征和生长特性.
- 识别了病毒与宿主相互作用网络和具有广泛宿主范围的前病毒,跨越细菌类.
- 病毒中的辅助代谢基因富含碳水化合物代谢功能.
- 实验数据证实了菌对海洋细菌的转录组的影响.
结论:
- 海洋 prokaryotes 经常被 lysogenized,表明广泛的病毒宿主集成.
- 海洋病毒扮演着重要的生态角色,影响宿主生理学,并可能调解家族间基因转移.
- 该研究为海洋病毒和 prokaryote 基因组学提供了一个全面的资源.
- 对病毒-宿主动态的进一步研究对于了解海洋生态系统的功能至关重要.
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