对应激应激的硫酸盐减少细菌的转录组范围的标记基因表达分析
Kalimuthu Jawaharraj1,2,3, Vincent Peta4, Saurabh Sudha Dhiman1,3,5
1Civil and Environmental Engineering, South Dakota Mines, 501 E. St. Joseph Street, Rapid City, SD, 57701, USA.
Scientific reports
|September 27, 2023
概括
减少硫酸盐的细菌 (SRB) 在环境循环和基础设施腐蚀中起着关键作用. 这项研究使用基因组和转录组分析解读了它们的应激反应机制,为预测深度学习工具铺平了道路.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 基因组学就是基因组学.
背景情况:
- 减少硫酸盐的细菌 (SRB) 在无氧生态系统中至关重要,并导致基础设施的重大生物腐蚀.
- 由于经济影响,了解环境压力下的SRB遗传机制至关重要.
研究的目的:
- 分析四种SRB物种的应激反应机制.
- 确定关键的转录特征和基因集群,参与SRB环境适应.
主要方法:
- 利用了全转录组标记物基因面板映射和基因聚类分析.
- 挖掘了公共RNA测序数据,并为Oleidesulfovibrio alaskensis产生了内部数据.
- 进行了比较基因组分析.
主要成果:
- 在Desulfovibrio spp.中确定了关键的转录候选基因. 和验证的基因集群预测.
- 揭示了 Oleidesulfovibrio alaskensis 在石墨烯和铜接口的独特转录特征.
- 对比基因组学显示,在四个SRB基因组中,有12821个转化基因.
结论:
- 这项研究提供了关于在压力下SRB遗传调节的见解.
- 为开发用于SRB基因调节分析的预测深度学习工具铺平了道路.
相关概念视频
Other Stress Responses in Bacteria
30
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
30
Stringent Response in E. coli
28
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
28


