22个翻译后修改的体碰撞截面
Andreas Will1, Denys Oliinyk1, Christian Bleiholder2
1Functional Proteomics, Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany.
Analytical and bioanalytical chemistry
|September 27, 2023
概括
翻译后修改 (PTMs) 在离子运动质谱学中改变碰撞截面 (CCS). 这些在CCS中的PTM和序列特异性变化为全蛋白质组的PTM分析提供了新的途径.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 分析化学 分析化学
- 生物化学 生化学
背景情况:
- 离子移动性越来越多地被用作基于质谱 (MS) 的蛋白质组学中的额外分离维度.
- 它根据气相中的大小和形状来分离离子.
- 了解后翻译修饰 (PTMs) 如何影响离子流动性对于推进蛋白质组分析至关重要.
研究的目的:
- 研究22种不同的PTM对的碰撞截面 (CCS) 的影响.
- 量化CCS变化对修饰与未修饰的变化.
- 探索PTMs,序列和气相结构之间的关系.
主要方法:
- 分析了大约4300对经过修改和未经修改的离子物种,使用被困离子移动性光谱学 (TIMS).
- 使用尖端参考酸进行线性对齐,以确保可重复的CCS测量 (中位变化系数为0.26%).
- 在不同的电荷状态中,对改性和未改性之间的CCS值进行比较.
主要成果:
- 修改的m/z与离子移动空间的观察到的再分配,受电荷状态变化的影响.
- 在同一个电荷状态的修饰和未修饰之间,确定了从-1.4% (素化) 到+4.5% (O-GlcNAcylation) 的中位数CCS转移.
- 发现虽然增加的质量通常与更高的CCS相关,但给定PTM的实际CCS变化是PTM和序列特定的,部分取决于未经修改的的气相结构.
结论:
- PTM对碰撞横截面产生显著和特定的影响.
- 这些PTM和序列特定的CCS变化可以被利用来改进全蛋白质组的PTM检测和表征.
- 这些发现为利用离子运动数据提供了基础,以更好地了解PTM在生物系统中的功能影响.
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