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开发一种使用向蛋白质组的ID-LC-MS/MS方法,用于在人血清中量化心脏素I

Meltem Asicioglu^1,2,3, Merve Oztug⁴, Nevin Gul Karaguler^2,3

¹TUBITAK National Metrology Institute (TUBITAK UME), Gebze, 41400, Kocaeli, Turkey.

Clinical proteomics

|September 27, 2023

概括

这项研究开发了一种标准化方法,用于测量血清中的心脏素I (cTnI),使用同位素稀释液体色谱-并联质谱. 新的测定方法确保了精确的cTnI量化,提高了急性心肌梗塞的诊断可靠性.

科学领域:

生物化学生物化学
分析化学分析化学
临床诊断临床诊断临床诊断

背景情况:

心脏素I (cTnI) 是急性心肌梗塞的一个关键生物标志物.
目前对cTnI的商业试验缺乏标准化,导致结果变化.
准确的cTnI测量对于可靠的诊断和患者管理至关重要.

研究的目的:

开发一种可追溯到国际单位系统的心脏素I (cTnI) 的测量程序.
建立一种标准化方法,用于精确量化cTnI在人血清中.
改善跨临床实验室cTnI结果的协调.

主要方法:

在磁纳米颗粒上对抗cTnI抗体的固定,以便从血清中分离cTnI.
使用素分离的cTnI的酶性消化.
通过同位素稀释液体染色学-并联质谱法 (ID-LC-MS/MS) 与蛋白质校准同时量化cTnI.

主要成果:

开发的方法显示了高线性 (R > 0.996) 对cTnI量化从0.7到24μg/L.
达到1.8μg/L的量化极限和0.6μg/L的检测极限.
展示了出色的精度 (可重复性≤8.7%) 和准确性 (90-110%) 与低测量不确定性 (≤12.5%).

结论:

这种分析方法可以准确量化人血清中的心脏素I.
这种标准化的方法可以在临床实验室中协调CTnI结果.
该方法支持可靠的cTnI测量和对参考材料的目标值的分配.

关键词:

心脏中位素 I 的心脏中位素 I 同位素稀释液体色谱学串联质谱学质谱学参考测量程序参考测量程序可追溯性可追溯性

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