亚纳等离子体 (Anaplasma phagocytophilum) Ats-1通过Syntenin-1增强了外体的分泌
Ruirui Li1,2,3, Zhongchen Ma1,2, Wei Zheng1
1International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi, China.
BMC microbiology
|September 27, 2023
概括
亚纳等离子体 fagocytophilum 效应器 Ats-1 劫持宿主合成蛋白-1 (SDCBP) 来操纵外体细胞的通路. 这种相互作用通过改变宿主细胞过程,增强了寄生虫感染和繁殖.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
背景情况:
- 亚纳等离子体细胞菌体会导致粒细胞性亚纳等离子体.
- 作用因子Ats-1是调节宿主细胞亡和新陈代谢的关键毒性因子.
- 呼吸链mPTP轴与Ats-1的调节功能有关.
研究的目的:
- 为了识别与A. phagocytophilum效应体Ats-1相互作用的宿主蛋白.
- 阐明Ats-1在调节宿主细胞外分泌中的作用.
- 了解A. 胞菌感染和扩散的细胞内机制.
主要方法:
- 酵母二杂交试验用于Ats-1结合蛋白的初始选.
- 免疫沉以确认Ats-1和syntenin-1 (SDCBP) 之间的相互作用.
- 生物信息学分析预测SDCBP在宿主途径中的作用.
- HEK293T细胞实验评估Ats-1和SDCBP对外体分泌的影响.
主要成果:
- Ats-1 与syntenin-1 (SDCBP) 相互作用,这是一种参与宿主外体细胞通路的蛋白质.
- 在SDCBP中,它与syndecans (SDC1,SDC2,SDC4) 相互作用.
- 通过SDCBP,Ats-1诱导SDC1,SDC2和SDC4的表达,增加宿主细胞中的外体分泌.
结论:
- SDCBP对于主体外体分泌的Ats-1-介导调节至关重要.
- 甲细胞利用SDCBP-syndecan轴来操纵宿主外体细胞路径.
- 这种操纵可能会提高寄生虫的生存,感染和宿主细胞内的增殖.
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