通过与双块共聚合物复合的双链RNA的有效传递和选择性杀虫活性因聚合物块组成而有所不同
Charlotte E Pugsley1,2, R Elwyn Isaac2, Nicholas J Warren1
1School of Chemical and Process Engineering, University of Leeds, Leeds, UK.
Pest management science
|September 28, 2023
概括
双块共聚合物有效地保护双链RNA (dsRNA) 进行RNA干扰 (RNAi) 害虫控制. 优化的聚合物显著降低了害虫的生存率,而不会伤害有益的昆虫,提供了特定物种的生物杀虫剂解决方案.
科学领域:
- 生物技术是生物技术.
- 昆虫学 昆虫学是一门学科.
- 材料科学 材料科学 材料科学
背景情况:
- 化学杀虫剂面临的挑战包括耐药性和缺乏特异性.
- 使用双链RNA (dsRNA) 的RNA干扰 (RNAi) 提供了特定物种的替代方案.
- 由于肠道降解和Drosophila suzukii等害虫的缓慢细胞吸收,dsrna的有效传递受到阻碍.
研究的目的:
- 开发和评估双块共聚合物作为害虫控制中dsRNA的输送载体.
- 评估dsrna对酶降解的保护,并增强细胞吸收.
- 使用这些传递系统来确定基于RNAi的害虫控制的有效性和物种特异性.
主要方法:
- 对dsRNA与定制的双块共聚合物的复合,以形成多复合体.
- 在体外评估dSRNA对肠道核酶酶酶降解的保护.
- 细胞流量计分析细胞吸收标记的dsRNA.
- 用D. suzukii幼虫进行口服养试验,以评估杀虫剂的有效性和毒性.
主要成果:
- 双阻塞共聚合物有效地保护dSRNA免受酶降解.
- 含有dsRNA的多重复合体显示细胞吸收增加了10倍.
- 一种特定的双块共聚合物配方 (1:2 阴离子/中性块) 显著降低了D. suzukii幼虫的存活率87%.
- 在密切相关的物种Drosophila melanogaster.中没有观察到毒性.
结论:
- 用二阻断共聚物复合dSRNA是一种基于RNAi的害虫控制的有前途的策略.
- 优化聚合物组成,特别是中性块长度,对于成功的RNAi至关重要.
- 这种方法为化学杀虫剂提供了一个潜在的物种特异性和环保的替代方案.
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