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In situ Hybridization for Sipunculus nudus Coelomic Fluid
Published on: May 1, 2020
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在花生 (Sipunculus nudus) 中的潮区不同环境的转录组反应
Junwei Li1,2, Jiufu Wen1,2, Ruiping Hu3
1South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
Biology
|September 28, 2023
概括
来自不同潮区的花生 (Sipunculus nudus) 对环境压力表现出不同的分子反应. 转录组分析揭示了不同的基因表达模式,表明了差异性影响,并突出了MAPK途径在免疫反应中的作用.
科学领域:
- 海洋生物学 海洋生物学
- 分子生态学分子生态学
- 环境基因组学 环境基因组学
背景情况:
- 螺 (Sipunculus nudus) 是一个具有全球意义的潮间物种.
- 潮间生物面临着不同的环境波动和影响.
- 了解分子反应对于水产养殖和保护至关重要.
研究的目的:
- 研究S. nudus.中环境应激反应的分子机制.
- 为了比较来自不同潮间区域的S. nudus的转录组概况.
- 为了确定关键的基因和途径参与压力适应.
主要方法:
- 组合的SMRT (太平洋生物科学单分子实时) 和Illumina测序用于转录组分析.
- 单基因的组合和差异表达基因 (DEGs) 的注释.
- KEGG途径分析以确定丰富的生物过程.
主要成果:
- 组装了105,259个单基因,其中有23,063个注释,显著扩大了S. nudus遗传数据库.
- 确定了830个DEG,其中33个与病原体,蛋白质合成和免疫反应相关的途径显著丰富.
- 在更加紧张的区域 (M和L) 观察到与病原体相关的DEGs (例如HSPA1,NFKBIA) 的更高表达,并在H区域上调MAPK通路基因 (例如MKP,HSPB1).
结论:
- 来自不同潮区的S. nudus种群经历着不同的环境压力.
- 该MAPK信号通路在免疫反应中发挥着关键作用,对不同的潮间条件有所不同.
- 这项研究为未来研究S. nudus环境应激适应的分子基础提供了基础.
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