S100A6蛋白与四螺旋式细胞因子的相互作用
Alexey S Kazakov1, Evgenia I Deryusheva1, Victoria A Rastrygina1
1Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institute for Biological Instrumentation, Institutskaya str., 7, Pushchino, Moscow Region 142290, Russia.
Biomolecules
|September 28, 2023
概括
蛋白质S100A6与广泛的四螺旋式细胞因子结合,类似于S100P. 这表明S100蛋白质在各种疾病中具有调节细胞因子活性的保留位置.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- S100蛋白质是参与许多生理和病理过程的多功能调节剂.
- 特定的S100蛋白,如S100P,可以与细胞因子相互作用,影响细胞信号传递.
- 通过特定的结合部位,S100P与大约71%的研究的四螺旋式细胞因子结合.
研究的目的:
- 为了研究S100A6蛋白与四螺旋式细胞因子的结合能力.
- 为了比较S100A6的细胞因子结合概况与S100P的概况.
- 阐明S100A6-细胞因子相互作用的分子基础.
主要方法:
- 使用表面等离子体共振光谱测量了复合人体S100A6与35种不同的四螺旋式细胞因子的结合亲和力.
- 进行了分子对接研究,以确定S100A6.6上的细胞因子结合部位.
- 在S100A6和S100P之间进行了序列识别分析.
主要成果:
- 在测试的35种四螺旋式细胞因子中,S100A6识别了26种 (约73%),其亲和度从0.3nM到12μM不等.
- 对于细胞因子,S100A6的结合选择性与S100P的结合选择性相似,但对互白素-2和哥斯塔丁M观察到较小的差异.
- 分子对接证实S100A6细胞因子结合部位与S100P.中发现的类似.
结论:
- 与S100P一样,S100A6具有广泛的特异性,可以结合四螺旋式细胞因子.
- 这些发现表明,乱交的S100家族成员可能共享一个保存的细胞因子结合部位.
- 这种保存的相互作用机制凸显了S100蛋白在过度释放细胞因子的疾病状态期间调节细胞因子活性方面的潜在作用.
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