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相关概念视频

Ribozymes02:47

Ribozymes

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The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Lysosomal Hydrolases01:22

Lysosomal Hydrolases

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Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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相关实验视频

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NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
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NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

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核糖核酸化酶 - 结构,功能,生理作用和实际用途

Leonid A Shaposhnikov1,2, Svyatoslav S Savin1,2, Vladimir I Tishkov1,2

  • 1Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences, Moscow 119071, Russia.

Biomolecules
|September 28, 2023
PubMed
概括
此摘要是机器生成的。

核糖核酸酸酶可以分解核糖核酸. 它们在大多数生物体中的确切功能仍然是未知的,但本次评论探讨了它们的不同类型,作用和应用.

关键词:
癌症药物设计 癌症药物设计催化机制是一种催化机制.晶体结构 晶体结构酶动力学 酶动力学核酸酸盐的核酸酸盐.

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科学领域:

  • 生物化学 生物化学
  • 酶学 是一种酶学.
  • 分子生物学分子生物学

背景情况:

  • 核酸酸酶催化了核酸的分裂成基和核糖.
  • 这些酶广泛分布在各种生命形式中,包括细菌,古生物,原生动物,超生动物,酵母,真菌和植物.
  • 在许多生物体中,核糖核酸酸酶的精确生理意义在很大程度上仍未确定.

研究的目的:

  • 审查和比较不同类型的核糖核酸酸酶.
  • 分析它们的结构特征,运动参数和生理作用.
  • 探索这些酶的潜在应用.

主要方法:

  • 文献综述和对 ribonucleoside hydrolases 的现有研究进行比较分析.
  • 检查有关酶结构,动力学和生物功能的数据.
  • 识别和讨论潜在的生物技术和治疗应用.

主要成果:

  • 有不同的核糖核酸酸酶家族存在,具有不同的结构和运动性质.
  • 有证据表明,它可能在核酸救援途径, purin代谢和细胞平衡中发挥作用.
  • 几种核糖核酸酸酶显示出在诊断,治疗和生物催化剂中的应用的前景.

结论:

  • 核酸酸酶是一种多功能类型的酶,具有重要的,但未被充分研究的生理功能.
  • 对它们的结构功能关系和生物学作用进行进一步的研究是有必要的.
  • 核糖核酸酸酶的独特催化能力为生物技术创新提供了有前途的途径.