相关实验视频

Updated: Jul 15, 2025

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

普列尼林-1-衍生循环RNAs:被忽视的表观遗传调节器,在阿尔茨海默病中具有各种功能

Nima Sanadgol¹, Javad Amini², Cordian Beyer¹

¹Institute of Neuroanatomy, RWTH University Hospital Aachen, 52074 Aachen, Germany.

|September 28, 2023

概括

从presenilin-1基因 (circPSEN1s) 衍生出的循环RNA可能通过菌微RNA和与蛋白质相互作用来保护阿尔茨海默病,从而影响TGF-β等关键信号通路.

科学领域:

分子生物学分子生物学
神经科学是一个神经科学.
遗传学遗传学是一个

背景情况:

阿尔茨海默病 (AD) 是一种进展性神经退行性疾病,与前列林-1 (PSEN1) 基因有关.
循环RNAs (circRNAs) 是由反向拼接形成的非编码RNA,它们在AD中的作用尚未完全理解.

研究的目的:

研究来自PSEN1基因的循环RNA (circPSEN1s) 和它们在阿尔茨海默病中的潜在功能.
探索AD中circPSEN1s影响的分子相互作用和信号通路.

主要方法:

在分析中识别circPSEN1s及其与微RNA (miRNA) 和蛋白质的相互作用.
途径分析以确定这些相互作用影响的生物过程.
分子对接以确认circPSEN1s,蛋白质和粉样β 42 (Aβ42) 之间的相互作用.
微阵列数据分析以检查阿尔茨海默病患者大脑中的基因表达模式.

主要成果:

确定了两个特定的circPSEN1s (hsa_circ_0008521和chr14:73614502-73614802),它们作为miRNA海绵.
发现circPSEN1s通过将miRNA向PI3K-Akt信号通路来保护特定的mRNA (例如QSER1,BACE2).
揭示了circPSEN1s与参与TGF-β和Notch信号通路的蛋白质的潜在相互作用.
通过分子对接,证实了circPSEN1s,枢纽蛋白和Aβ42之间的相互作用.
在AD大脑中观察到circPSEN1受影响基因的独特表达模式.

结论:

通过调节miRNA和蛋白相互作用,circPSEN1s可能在AD的发病过程中发挥作用,影响关键的细胞信号通路,特别是TGF-β.
需要进一步的研究来阐明circPSEN1s在阿尔茨海默病中的确切机制和意义.

关键词:

阿尔茨海默氏症是阿尔茨海默氏症的一种疾病.在PSEN1上.圆形的RNAs是圆形的RNAs.在分析中,分析.这种miRNA的海绵化.

更多相关视频

Imaging the Intracellular Trafficking of APP with Photoactivatable GFP

Imaging the Intracellular Trafficking of APP with Photoactivatable GFP

Published on: October 17, 2015

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

相关概念视频

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...