尖端蛋白S1的N-葡萄糖概况:来自SARS-CoV-2的11种变体的系统和比较分析
Cristian D Gutierrez Reyes1, Sherifdeen Onigbinde1, Akeem Sanni1
1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.
Biomolecules
|September 28, 2023
概括
SARS-CoV-2 变种在 S1 蛋白中显示出明显的 N-糖体变化,影响病毒病变. 欧米克朗变体表现出增加的sialofucosylated glycans,而感兴趣的变体显示出更多的fucosylated glycans.
科学领域:
- 病毒学 病毒学
- 葡萄糖生物学 葡萄糖生物学
- 结构生物学 结构生物学
背景情况:
- SARS-CoV-2 尖端蛋白 S1 子单元对于病毒进入至关重要,也是治疗药物和疫苗的关键目标.
- 糖化S1蛋白质的糖化通过屏蔽病毒的活性部位,在免疫逃避中发挥作用.
- 了解SARS-CoV-2变种中的甘氨酸变化对于理解病原和进化至关重要.
研究的目的:
- 在11种SARS-CoV-2变种中调查和比较S1蛋白的N-糖化样本.
- 分析关注变体 (VOC) 和感兴趣变体 (VOI) 之间的甘氨酸表达差异.
- 为了将甘氨酸变化与对病毒病原发生的潜在影响相关联.
主要方法:
- 从11种SARS-CoV-2变种 (5种VOC,6种VOI) 的S1蛋白上的N-糖基化进行比较分析.
- 基于质谱的糖性分析.
- 量化和结构性表征N-glycans. 的量化和结构性表征.
主要成果:
- 在所有11种SARS-CoV-2变种中观察到N-糖丰度的显著变化.
- 关注的变种,特别是Omicron,显示了基基基的显着增加.
- 有趣的变体主要显示了丰富的fucosylated glycans.
- 两种特定的甘氨酸结构,GlcNAc4,Hex5,Fuc,NeuAc和GlcNAc6,Hex8,Fuc,NeuAc,在所有变体中都始终丰富.
结论:
- SARS-CoV-2 变种在 S1 蛋白上表现出明显的 N-糖体特征.
- 观察到的甘氨酸变化,特别是像Omicron这样的VOC中增加的基化,可能会影响病毒病原和免疫逃避.
- 这些发现有助于理解结构变异及其对SARS-CoV-2演变的影响.
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