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通过氧化物代谢物化学发光测试的样品处理引入的文物

Taiming Liu¹, Meijuan Zhang¹, Abraham Duot¹

¹Department of Pediatrics, Loma Linda University School of Medicine, Loma Linda, CA 92354, USA.

Antioxidants (Basel, Switzerland)

|September 28, 2023

概括

样品处理显著改变使用化学发光测试的氧化 (NOx) 测量结果. 适当的样本准备至关重要,以避免在检测亚酸盐,S-nitrosothiols,heme-nitrosyl和dinitrosyl铁复合物的过程中发现人工物.

科学领域:

生物化学生物化学
分析化学分析化学
生理学生理学生理学

背景情况:

氧化 (NO) 的代谢物,包括化物,S-化 (SNOs),血-化 (heme-NO) 和丁酸铁复合物 (DNIC),是重要的信号分子.
化学发光测试通常用于NOx检测,但样品处理的潜在工件尚未得到充分理解.

研究的目的:

研究各种样品处理程序对使用化学发光测试精确测量不同氧化 (NO) 代谢物 (NOx) 的影响.
为了测试样本处理在NOx量化中引入了重大人工物,这一假设.

主要方法:

使用来自低空和高空的胎儿和母羊作为不同NOx水平的模型.
评估了室温储存,快速冷/解,均质化,特定的"停止解决方案",脱蛋白化和常见试剂预处理对NOx稳定性的影响.
评估了血液成分对酸盐检测的灭效应.

主要成果:

室温储存改变了血和全血中的NOx水平.
快速冷和解诱导了血中NOx物种的相互转换.
快速冷和均质化减少了大动脉样本中的NOx.
"停止解决方案"和脱蛋白化导致NOx相互转换和分别增加可检测的NOx.
化和EDTA等试剂破坏了各种NOx物种的稳定.
血液成分灭了亚酸盐信号.

结论:

样品处理程序在基于化学发光的NOx测定中引入了重要的工件.
以前的NOx测量可能需要重新评估,因为可能会因处理引起的变化.
严格关注样品处理协议对于可靠的NOx量化至关重要.

关键词:

在DNIC中,它是DNIC.NO 的代谢物 NO 的代谢物.这是一个陷,陷.这就是S-nitrosothiol.化学发光方法的方法学.血红 - 没有.亚酸盐的化物

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