相关实验视频

Updated: Jul 15, 2025

Assessing Microglial Phagocytosis of Myelin Debris in vitro Under Repeated Magnetic Stimulation

Assessing Microglial Phagocytosis of Myelin Debris in vitro Under Repeated Magnetic Stimulation

Published on: June 17, 2025

高频重复磁刺激通过调节p62/Keap1/Nrf2和p38 MAPK通路激活了巨细胞的杀菌活性

Therese B Deramaudt¹, Ahmad Chehaitly¹, Théo Charrière¹

¹U1179 INSERM, END-ICAP, UFR des Sciences de la Santé-Simone Veil, Université de Versailles Saint-Quentin-en-Yvelines, 78180 Montigny-le-Bretonneux, France.

Antioxidants (Basel, Switzerland)

|September 28, 2023

概括

重复磁刺激 (rMS) 激活了巨细胞中的Nrf2通路,增强了它们对抗金黄色葡萄球菌感染的能力. 这种免疫调节为细菌防御提供了一种新的治疗方法.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
神经科学是一个神经科学.

背景情况:

重复磁性刺激 (rMS) 的效应主要研究在易刺激的细胞中.
有限的研究存在于rMS对巨细胞等不可激发细胞的影响.
巨细胞是先天免疫防御的关键参与者.

研究的目的:

研究rMS对巨细胞功能的影响.
确定rMS是否可以增强巨细胞对细菌病原体的防御.
探索巨细胞中rMS的潜在分子机制.

主要方法:

从THP-1衍生出来的巨细胞在5分钟内受到10Hz的rMS.
分析了Nrf2激活,Keap1表达和p62酸化.
评估了黄金葡萄球菌 (Staphylococcus aureus) 的细胞内生存率.
使用Nrf2沉默 (siRNA) 和淘汰性巨细胞来确认Nrf2依赖性.
测量了炎症性基因表达 (IL-1β,TNF-α) 和p38 MAPK激活.

主要成果:

rMS增加了Nrf2激活,并降低了巨细胞中的Keap1表达.
Nrf2通路的激活取决于rMS诱导的p62酸化.
rMS显著降低了细胞内黄金葡萄球菌的存活率.
Nrf2沉默或淘汰消除了rMS的抗菌作用.
rMS减弱了S. aureus诱导的IL-1β和TNF-α的表达,并抑制了p38 MAPK的激活.

结论:

rMS激活了巨细胞中非正规的Nrf2通路.
rMS调节巨细胞功能,增强宿主对细菌感染的防御能力.
rMS显示出作为一种治疗策略来对抗细菌病原体的潜力.

关键词:

凯普1号码是什么意思 Nrf2 没有任何问题 Nrf2 淘汰赛小鼠黄金葡萄球菌黄金葡萄球菌由THP-1衍生的巨细胞.膜细胞巨细胞 p38 MAPKK 的一个.在P62上,P62就是P62 重复的磁性刺激是重复性的磁性刺激.

更多相关视频

Bone Marrow-derived Macrophage Production

Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

相关概念视频

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...

Immune Surveillance by NK Cells and Phagocytes

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.