相关实验视频

Updated: Jul 15, 2025

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Published on: January 1, 2017

DDAH1 保护心脏毒素诱导的肌肉损伤和再生

Fei Feng¹, Bingqing Cui², Li Fang³

¹School of Exercise and Health, Shanghai University of Sport, Shanghai 200438, China.

Antioxidants (Basel, Switzerland)

|September 28, 2023

概括

双甲基氨酸二甲基氨基酶1 (DDAH1) 保护肌肉免受损伤,并有助于再生. 它的缺乏会使肌肉损伤恶化,并通过增加炎症和氧化应激延迟恢复.

科学领域:

肌肉生物学肌肉生物学
分子信号传递是分子信号传递.
再生医学是一种再生医学.

背景情况:

氧化 (NO) 是肌肉再生中的关键信号分子.
双甲基氨基酸二甲基氨基酶1 (DDAH1) 通过降解不对称的双甲基氨基酸 (ADMA) 来调节NO合成酶 (NOS) 的活性.

研究的目的:

研究DDAH1在骨肌损伤和再生中的作用.
阐明DDAH1在肌肉修复中的功能背后的分子机制.

主要方法:

使用肌肉特定的DDAH1-Knockout (Ddah1^MKO) 和控制 (Ddah1^f/f) 鼠标.
使用心脏毒素 (CTX) 注射诱导肌肉损伤.
评估肌肉损伤,再生,炎症,氧化应激和亡.

主要成果:

与对照组相比,Ddah1^MKO小鼠在CTX后表现出更严重的肌肉损伤和延迟的再生.
在Ddah1^MKO小鼠中观察到较高的血清ADMA和LDH水平.
DDAH1缺乏导致受伤肌肉的炎症增加,氧化应激和亡,改变关键蛋白质表达 (例如,减少M-cadherin,myogenin,Bcl-2;增加MyoD,TNFα,Il-6,iNOS,Bax).

结论:

DDAH1在骨肌损伤和再生中起着至关重要的保护作用.
DDAH1 缺乏会加剧肌肉损伤,并通过炎症和氧化途径阻碍恢复.

关键词:

DDAH1 DDAH1 的时间心脏毒素是什么心脏毒素肌肉再生肌肉再生氧化应激是一种氧化应激.

更多相关视频

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Published on: September 18, 2019

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

相关概念视频

Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin

Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin

Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...

Satellite Stem Cells and Muscular Dystrophy

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...

Cardiomyopathy II: Dilated Cardiomyopathy

Cardiomyopathy II: Dilated Cardiomyopathy

Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...

Heart Failure Drugs: Inotropic Agents

Heart Failure Drugs: Inotropic Agents

Positive inotropic agents are commonly used as the first line of treatment for heart failure. One such agent is digoxin, derived from the genus Digitalis, which has been known for centuries but effectively utilized since 1785. However, these cardiac glycosides can have potentially toxic effects due to their mechanism of action, which involves inhibiting Na+/K+-ATPase and increasing contractility. Digoxin is absorbed orally and distributed in various tissues, including the CNS. It has a long...