增强人类多能干细胞的扩张和体细胞重编程,使用定义和无异种培养条件

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

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Suraj Timilsina¹, Kaitlyn Faith McCandliss², Evan Trivedi³

¹Department of Biomarkers and Investigative Pathology Unit (BIPU), Charles River Laboratories, Mattawan, MI 49071, USA.

Bioengineering (Basel, Switzerland)

|September 28, 2023

概括

研究人员为人类多能干细胞 (hPSC) 开发了化学定义的,无外基因培养条件. 这种方法增强了细胞的附着和扩张,这对于再生医学应用至关重要.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
发展生物学发展生物学

背景情况:

人类多能干细胞 (hPSC) 对发育生物学和再生医学具有前景.
目前的应用受限于缺乏化学定义和无外源培养条件.
可扩展,高质量的细胞生产需要简化体外培养方法.

研究的目的:

为了确定hPSC初始附着的最佳条件,使用PMEDSAH移植的盘子和定义的介质.
为hPSC的维护和扩展开发一种化学定义和无异种培养系统.

主要方法:

对补充剂和化学品进行系统测试,以便将hPSC固定在PMEDSAH移植板上.
细胞附着和长期培养与Matrigel涂层板的比较.
评估细胞扩张,染色体稳定性和重编程效率.

主要成果:

预先调节PMEDSAH板,使用10%的人类血清 (HS) 优化初始hPSC附着.
这种方法使长期的hPSC培养和维护成为可能,优于Matrigel.
在没有染色体异常的情况下实现了hPSC扩张的2.1倍增加.
将体细胞重新编程成诱导多能干细胞 (iPSC) 的效率有所提高 (0.37%对比0.22%).

结论:

使用PMEDSAH板和HS预条件的定义,无异种培养条件支持强大的hPSC扩张.
这一系统有助于产生大量的hPSC和患者衍生的iPSC.
开发的方法适用于再生和转化医学中的应用.

关键词:

在PMEDSAH的基础上.人类胚胎干细胞人类多能干细胞干细胞重编程是重新编程.它是自我更新的.合成聚合物接种合成聚合物接种

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