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中红外手持和长板光谱仪的比较,用于检测骨移植中的Staphylococcus epidermidis

Richard Lindtner¹, Alexander Wurm^1,2, Katrin Kugel¹

¹Department of Orthopaedics and Traumatology, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, Austria.

Bioengineering (Basel, Switzerland)

|September 28, 2023

概括

中红外光谱学有效地使用手持式和长板仪器诊断Staphylococcus epidermidis骨感染. 这两种方法都能检测到骨质量损失,为快速分析提供了宝贵的工具.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
传染性疾病传染性疾病

背景情况:

中红外光谱 (MIR) 越来越多地用于骨分析.
手持式光谱仪提供现场测试能力,但数据质量至关重要.
诊断骨移植中的细菌感染对于患者的治疗结果至关重要.

研究的目的:

为了比较一个手持的富里埃变换红外光谱仪 (FTIR) 与一个台式FTIR仪器的诊断效率,用于检测人类骨移植中的Staphylococcus epidermidis.
评估使用MIR光谱技术用于快速,现场骨感染诊断的可行性.

主要方法:

使用MIR光谱学分析了40个非感染者和10个感染者的人类骨样本.
在Agilent 4300手持FTIR和Perkin Elmer Spectrum 100减弱总反射 (ATR) FTIR仪器之间进行比较.
采集光谱数据 (650-4000 cm-1),并使用主要成分分析 (PCA) 进行分析.

主要成果:

手持式和台式FTIR仪器在检测Staphylococcus epidermidis方面取得了显著的结果.
该研究发现,与感染相关的骨质质量明显下降.
频谱和无监督分类方法在诊断方面被证明是有效的.

结论:

特别是手持设备的MIR光谱是诊断骨感染的宝贵工具,当组织有限且时间至关重要时.
建议对更大的样本进行进一步研究,以充分验证该方法的优点和局限性.
现场MIR光谱可以帮助及时诊断和治疗骨感染.

关键词:

斯塔菲洛科克斯 (Staphylococcus epidermidis) 是一种表皮菌.减弱的总反射率光谱学.骨质骨质的质量骨质的质量手持式FTIR光谱仪是一种手持式FTIR光谱仪.主要组件分析主要组件分析

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