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在非均环境中对电场分布的有限元评估
Elisabetta Sieni1,2, Monica Dettin3, Annj Zamuner4
1Department of Theoretical and Applied Sciences, University of Insubria, Via Dunant 3, 21100 Varese, Italy.
Bioengineering (Basel, Switzerland)
|September 28, 2023
概括
介质的不均性在电穿孔过程中显著影响电场和细胞膜潜力. 增加细胞聚合和原的存在增强了跨膜潜力,提高了电穿孔效率.
科学领域:
- 生物物理学的生物物理.
- 计算生物学 计算生物学
- 生物材料是一种生物材料.
背景情况:
- 电穿孔是细胞操纵的一个关键技术.
- 了解复杂生物介质中的电场分布对于优化电穿孔至关重要.
- 细胞微环境,包括细胞外矩阵 (ECM) 组成和细胞密度,影响电穿孔结果.
研究的目的:
- 调查介质不均对高脉冲电场暴露期间电场分布和跨膜潜力的影响.
- 分析不同程度的细胞聚合和细胞外矩阵组成 (原,菌素矩阵) 如何影响细胞膜潜力.
- 用实验数据验证数值模拟结果.
主要方法:
- 用有限元分析 (FEA) 来建模电场分布.
- 一个具有可变细胞-细胞距离 (1-283μm) 的代表性模型模拟了不均性.
- 细胞外介质的导电性是使用原蛋白,myxoid矩阵和它们的组合而变化的.
- 在电穿孔条件下计算了跨膜电位.
- 模拟被验证使用HCC1954细胞培养在基于氨酸的支架.
主要成果:
- 较高的细胞聚合导致了增加的跨膜潜力.
- 细胞聚合物和原的存在显著影响了跨膜潜力.
- 增加的细胞聚合,在原和状基质条件下,导致更高的跨膜潜力.
- 实验验证证证实,原体的存在在较低电场强度下增强了电穿孔.
结论:
- 介质的不均性,特别是细胞聚合和原含量,在电穿孔过程中对调节电场分布和跨膜潜力的作用至关重要.
- 在复杂的生物环境中,FEA是预测电穿孔下的细胞行为的一个有价值的工具.
- 这些发现表明,优化ECM组成和细胞密度可以提高治疗应用的电穿孔效率.
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