供体动物生产阶段的差异会影响体外发酵发酵动力学的重复性
Britt Jantzen1, Hanne Helene Hansen1
1Department of Veterinary and Animal Sciences, University of Copenhagen, Grønnegårdsvej 3, 1870 Frederiksberg C, Denmark.
Animals : an open access journal from MDPI
|September 28, 2023
概括
实验室气体生产技术 (IVGPT) 对甲产量的重复性很好,但气体生产曲线和pH值存在变化,这取决于液的来源. 重复性受捐赠者类型的影响.
科学领域:
- 动物科学动物科学
- 类动物营养 类动物营养
- 在玻璃发酵技术.
背景情况:
- 试验室气体生产技术 (IVGPT) 对于评估料和添加剂,减少动物使用和提高动物福利至关重要.
- 关于IVGPT可重复性的数据有限,这阻碍了其广泛采用和解释.
研究的目的:
- 为了评估IVGPT参数的变化,在单个实验室环境中使用不同类型的供体 (母乳母牛和母牛) 的液.
- 评估关键发酵参数的重复性,包括气体生产,甲,pH和干物质降解.
主要方法:
- 在一年内,使用一致的料基板进行了17次24小时的体外发酵.
- 乳液来源于两种不同类型的捐赠者:乳母奶牛和禁食小牛.
- 分析的关键参数包括气体曲线特征,总气体产量 (TGP),甲度和产量,pH值和降解干物质 (dDM).
主要成果:
- 在捐赠者类型之间观察到液和发酵液pH值的显著差异,但在类型内没有.
- 甲度和24小时后的产量在供体类型之间或供体类型内没有显著差异.
- 早期的气体生产率在供体类型之间存在差异,而24小时的基线校正后的TGP和降解干物 (dDM) 没有显著差异.
- 气体生产曲线在供体类型之间差异很大,表明不同的发酵模式.
结论:
- 虽然IVGPT为甲产量提供了良好的可重复性,但气体产量曲线和pH值的变化需要仔细考虑供体类型.
- IVGPT的重复性受捐赠动物的生理状态和生产阶段的影响.
- 建议对标准化供体动物选择进行进一步的研究,以提高IVGPT的可靠性.
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