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亚临床乳腺炎导致多种药物耐药性黄金葡萄球菌的遗传和表型表征

Amanda Thaís Ferreira Silva¹, Juliano Leonel Gonçalves², Stéfani Thais Alves Dantas^3,4

¹Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife 52171-900, Brazil.

Antibiotics (Basel, Switzerland)

|September 28, 2023

概括

这项研究在奶牛中特征了多药耐药黄金葡萄球菌 (MDRSA),确定了亚临床乳腺炎的危险因素. 研究结果强调需要了解MDRSA菌株分布和耐药性概况,以便制定有效的预防策略.

科学领域:

兽医微生物学兽医微生物学
抗微生物耐药性抗微生物耐药性
牛的健康问题

背景情况:

乳牛的亚临床乳腺炎是一个重要的经济问题.
多药耐药黄金葡萄球菌 (MDRSA) 对动物和公共健康构成越来越大的威胁.
对MDRSA及其相关风险因素的表征对于有效控制至关重要.

研究的目的:

为了在遗传和表型上表征导致乳牛亚临床乳腺炎的MDRSA.
调查与由MDRSA引起的亚临床乳腺炎相关的风险因素.
确定巴西乳制品农场中MDRSA的流行率和遗传多样性.

主要方法:

来自牛奶,餐具和奶器的细菌培养.
用于识别的矩阵辅助激光脱/离子化飞行时间质谱 (MALDI-TOF MS).
抗微生物耐药性查,生物膜检测,基因检测,脉冲场凝电泳 (PFGE) 和多位置序列类型 (MLST).

主要成果:

188个黄金葡萄球菌分离物被发现;7%是MDRSA.
36%的MDRSA分离物是抗塞福西的,但缺乏mecA/mecC基因.
PFGE表明了群体特定的菌株;确定了CC97 (ST126) 和CC1 (ST7440). 在巴西,在牛乳炎中新报告了ST7440病例.
均性,哺乳期,SCC,牛奶产量和牛群大小是重要的风险因素.

结论:

奶牛群中的MDRSA菌株表现出不同的遗传特征和耐药性机制.
了解MDRSA的分布和抗菌素耐药性对于制定有针对性的预防和控制策略至关重要.
识别新型MDRSA菌株和相关风险因素为改善奶牛群管理和减少抗菌素耐药性传播提供了关键数据.

关键词:

黄金葡萄球菌黄金葡萄球菌抗生素耐药性抗生素耐药性在乳腺内感染.分子流行病学分子流行病学

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