横向基因转移和药物耐药性 涉及 Mycobacterium 结核病
Xuhua Xia1,2
1Department of Biology, University of Ottawa, Ottawa, ON K1N 9A7, Canada.
Antibiotics (Basel, Switzerland)
|September 28, 2023
概括
横向基因转移 (HGT) 在Mycobacterium tuberculosis (Mtb) 中发生,但不会导致抗生素耐药性. 在Mtb中,耐药性源于Mtb复合体内的突变,而不是通过HGT获得基因.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 结核菌 (Mycobacterium tuberculosis) (Mtb) 呈现出快速获得抗药性,这是其缓慢的复制率而异常的.
- 假设水平基因转移 (HGT) 解释了Mtb的快速药物耐药性演变.
- 研究Mtb和其他真菌细菌之间的HGT对于理解耐药机制至关重要.
研究的目的:
- 为了确定HGT是否发生在Mtb和其他真菌细菌物种之间.
- 为了确定哪些基因在受体基因组中在HGT后被保留.
- 评估HGT对MTB抗生素耐药性的贡献.
主要方法:
- 对43个Mycobacterium基因组的分析,重点是核糖体蛋白质基因,rRNA基因和抗生素向基因.
- 包括mgtC和插入序列IS6110,此前与HGT.相关.
- 开发一个概念框架来检测HGT事件.
主要成果:
- 发现了涉及Mtb复合体的HGT证据,特别是与插入序列IS6110.0的证据.
- 没有观察到功能性重要基因 (核糖体蛋白,rRNA,药物点) 通过HGT.被转移.
- 基因可转移性取决于基因功能,对功能关键基因进行选择.
结论:
- HGT发生在Mycobacterium属内,但似乎不是Mtb.抗生素耐药性的重要驱动因素.
- 在Mtb中抗生素耐药性主要来自Mtb基因组内的内在突变.
- 该研究强调了差异性选择在塑造不同基因类型的HGT事件可见性的作用.
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