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基于质谱分析的SARS-CoV-2变种的歧视性识别

Liron Feldberg¹, Anat Zvi², Yfat Yahalom-Ronen³

¹Department of Analytical Chemistry, Israel Institute for Biological Research (IIBR), Ness Ziona 74100, Israel.

|September 28, 2023

概括

一种新的基于质谱 (MS) 的方法可以快速识别SARS-CoV-2变种. 该技术使用独特的标记物进行变异特异性诊断,为检测有关菌株提供了更快的基因测序替代方案.

科学领域:

病毒学病毒学
分析化学分析化学
生物技术是生物技术.

背景情况:

在SARS-CoV-2关注变种 (VOCs) 中的遗传突变可以增加传播能力,疾病严重程度和免疫逃避,影响诊断和疫苗疗效.
目前用于变体歧视的金标准基因测序是耗时且昂贵的.
快速和具体的诊断方法对于控制SARS-CoV-2VOCs的传播至关重要.

研究的目的:

开发和验证基于质谱 (MS) 的快速,经济高效的方法论,用于对SARS-CoV-2的普遍识别和变异特异性歧视.
建立一种基于MS的诊断概念证明,使用代表性的VOC.

主要方法:

开发一种基于MS的方法来诊断SARS-CoV-2.
用于普遍检测SARS-CoV-2的保存的识别.
从尖端 (S) 和核体 (N) 蛋白质中鉴定变异特异性的三性.
细胞培养的SARS-CoV-2变体的三维消化和HR-LC-MS/MS分析.
对野生型武汉参考菌株进行验证.

主要成果:

基于多发性硬化症的方法成功识别了SARS-CoV-2的普遍性,并对特定变体进行了歧视.
至少两种独特的标记物,主要来自S和N蛋白质,被确定为每个测试的VOC.
该方法证明了其特异性,快速性,易于执行和成本效益.

结论:

开发的基于MS的方法提供了一个快速,具体和廉价的工具来诊断SARS-CoV-2并区分致病变体.
这种方法为变种监测和诊断提供了传统测序的可行替代方案.

关键词:

在 COVID19 疫情下,这是LC-MS/MS.这就是SARS-CoV-2病毒.诊断诊断诊断诊断诊断诊断质谱学质谱学是指质谱学.它是核体的核体.斯派克斯派克斯派克斯就是一个尖刺.有关关注的变种.

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