离子化度分析剂的稳定性及其在健康志愿者的参考范围
Juhaina Salim Al-Maqbali1,2, Abdullah M Al Alawi3,4, Zubaida Al-Falahi3,4
1Department of Pharmacology and Clinical Pharmacy, College of Medicine and Health Science, Sultan Qaboos University, Muscat 123, Oman.
Biomedicines
|September 28, 2023
概括
在2-8°C下冷却电离 (iMg) 样品长达1小时,与新鲜样品保持出色的相关性. 这一发现支持使用短期冷藏作为iMg分析的可行替代方案.
科学领域:
- 临床化学 临床化学
- 生物化学 生物化学
- 实验室医学 实验室医学
背景情况:
- 准确的电离 (iMg) 测量对于临床诊断至关重要.
- 建立可靠的参考范围和样品稳定性协议对于实验室测试至关重要.
研究的目的:
- 为了评估冷藏血液样本中电离 (iMg) 度的稳定性.
- 在健康的阿曼成年人群中建立IMg参考范围.
- 为了确定样品冷却的最佳时间框架,而不会影响iMg测量准确度.
主要方法:
- 用于IMg度测量的直接离子选择性电极技术.
- 用了皮尔森和林的一致性相关系数,以及布兰德-阿尔特曼图用于一致性分析.
- 这项研究涉及167名健康的阿曼志愿者 (≥18岁).
主要成果:
- 确定的新鲜的iMg参考范围:中位数0.55,第2.5百分位数0.47,第97.5百分位数0.68 mmol/L.
- 新鲜和冷藏样品之间的总体一致性很差 (rho-c = 0.51).
- 在冷藏时间 ≤1小时的样品中观察到优异的相关性 (林氏rho-c = 0.80) 和最小偏差 (0.009 mmol/L).
结论:
- 在2-8°C下冷却截止期≤1小时,可为IMg的立即分析提供可接受的替代方案.
- 这一发现为IMG测试的样本处理提供了灵活性,可能改善实验室的工作流程.
- 该研究成功地为阿曼成年人建立了IMg参考范围.
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