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在缺血性脑卒中模型中,人类诱导的多能干细胞衍生中介细胞干细胞细胞外囊的神经保护作用

Gang Lu^1,2,3,4, Xianwei Su¹, Lihong Wang¹

¹CUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China.

|September 28, 2023

概括

来自干细胞的细胞外囊泡保护大脑细胞,并减少小鼠中风损伤. 这表明一种潜在的无细胞疗法用于缺血性中风恢复.

科学领域:

神经科学是一个神经科学.
再生医学是一种再生医学.
生物技术是生物技术.

背景情况:

脑卒中是导致死亡和残疾的主要原因.
中介细胞干细胞 (MSC) 移植显示出大脑缺血恢复的前景.
从人类诱导的多能干细胞衍生MSC (hiPS-MSC-EVs) 来研究细胞外囊泡 (EVs) 进行神经保护.

研究的目的:

在缺血性中风的临床前模型中评估hiPS-MSC-EVs的神经保护潜力.
评估hiPS-MSC-EVs对细胞存活率和中风结果的 in vitro 和 in vivo 的影响.

主要方法:

通过超过分离的hiPS-MSC-EVs.
在氧气-葡萄糖剥夺/再氧化 (OGD/R) 损伤后,用hiPS-MSC-EVs治疗的HT22细胞.
中脑动脉封闭 (MCAO) 的小鼠模型被静脉注射hiPS-MSC-EVs治疗.

主要成果:

hiPS-MSC-EVs增强了HT22细胞增殖,并减少了OGD/R后的亡.
在MCAO小鼠中,hiPS-MSC-EVs降低了心脏病发作体积和改善了运动功能.
在接受hiPS-MSC-EVs治疗的MCAO小鼠中,血管新生增强,VEGF和CXCR4表达增加.

结论:

在体外和体外缺血性中风模型中,hiPS-MSC-EVs表现出显著的神经保护作用.
这些发现支持hiPS-MSC-EVs作为神经恢复的潜在治疗剂.
结果强调了使用hiPS-MSC-EVs治疗大脑缺血症的无细胞策略的前景.

关键词:

血管新生是因为血管新生.外基因组是外基因组的组成部分.这就是hiPS-MSC-EVV.缺血性中风中风中脑动脉封闭中脑动脉封闭它们的扩散和扩散.

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