波马斯通过改善肠道屏障和调节肠道微生物群来缓解DSS诱导的大肠炎
Baixi Zhang1,2, Xinwen Huang1, Lijuan Niu1
1School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Foods (Basel, Switzerland)
|September 28, 2023
概括
通过改善肠道屏障功能和有益细菌,Lonicera caerulea (LCP) 补充剂在小鼠中减少了结肠炎症状. 液体消化显示出促进肠道健康的潜力.
科学领域:
- 胃肠病学 胃肠病学
- 营养科学 营养科学
- 微生物学 微生物学
背景情况:
- 炎症性肠道疾病,如大肠炎,对健康构成重大挑战.
- 果实加工的副产品,如Lonicera caerulea (LCP) 果汁,富含生物活性化合物.
- 了解LCP在肠道健康中的治疗潜力至关重要.
研究的目的:
- 在小鼠模型中评估LCP补充饮食对二硫酸 (DSS) 诱导的大肠炎的影响.
- 调查LCP的抗炎和肠道保护性能的潜在机制.
主要方法:
- 在小鼠中使用DSS诱导大肠炎.
- 用LCP补充饮食的管理.
- 评估临床大肠炎得分,结肠长度和组织病理学.
- 测量髓氧化酶 (MPO) 活性和炎症性细胞因子.
- 对肠道屏障蛋白表达的分析 (密集结蛋白,Muc2).
- 肠道微生物组成分析 (16S rRNA测序).
- 短链脂肪酸 (SCFA) 的量化.
主要成果:
- 补充LCP显著减轻了结肠炎症状,包括减少疾病活动指数 (DAI),更长的结肠长度和改善了他的病理学.
- LCP降低了MPO活性,并在结肠中抑制了前炎性细胞因子的表达.
- 通过上调紧密结蛋白和Muc2表达,LCP增强了肠道屏障的完整性.
- LCP调节了肠道微生物群,增加了α多样性,促进了有益的细菌 (Akkermansia,Coprococcus,Bifidobacterium),同时减少了致病细菌.
- 饮食中的LCP增加了肠道中的SCFA度.
结论:
- 在DSS诱导的大肠炎中,LCP表现出显著的抗炎和肠道保护作用.
- 低皮促进肠道屏障的修复,并有利地调节肠道微生物群的组成.
- 补充LCP是治疗大肠炎和促进肠道健康的有希望的饮食策略.
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