基于UPLC-Q-Orbitrap-MS,HS-SPME-GC-MS和HS-GC-IMS的木生长过程中的动态变化的探索
Shufang Wu1,2, Jiaxin Yin1,2, Xuejuan Li1,2
1College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Foods (Basel, Switzerland)
|September 28, 2023
概括
这项研究跟踪了在生长过程中中的非挥发性和挥发性有机化合物 (VOC). 黄和酸水平随着成熟而增加,在第四阶段抗氧化活性达到顶峰.
科学领域:
- 农业科学 农业科学
- 食品化学 食品化学
- 分析化学 分析化学
背景情况:
- 树的质量受到生长过程中有机化合物的动态变化的影响.
- 了解这些组成变化对于优化种植和加工至关重要.
研究的目的:
- 为了研究不同生长阶段在木中的非挥发性有机化合物 (n-VOCs) 和挥发性有机化合物 (VOCs) 的动态变化.
- 为了确定与木成熟和抗氧化活性相关的关键化合物.
主要方法:
- 使用超高性能液体染色学-四极-轨道轨道-质谱学 (UPLC-Q-轨道轨道-MS),头空间-固相微提取-气体染色学-质谱学 (HS-SPME-GC-MS) 和头空间-气体染色学-离子运动谱学 (HS-GC-IMS).
- 采用多变量统计分析,包括主要组件分析 (PCA),随机森林分析 (RFA) 和正交部分最小平方差分分析 (OPLS-DA).
主要成果:
- 总共发现了166种化合物 (68种n-VOC,98种VOC).
- 观察到74种化合物在成熟过程中出现或消失,其中24种持续存在.
- 黄和酸随着成熟而增加,与第四阶段增强的抗氧化活性相关.
结论:
- 建立了一种有效的分析策略,用于对木的动态成分分析.
- 结果支持动态变化过程分析和木的质量控制.
关键词:
在HS-GC-IMS中使用.在HS-SPME-GC-MS中使用.这就是UPLC-Q-Orbitrap-MS.动态过程分析 动态过程分析毛树是什么意思 毛树是什么意思不易挥发的有机化合物 不易挥发的有机化合物挥发性有机化合物 挥发性有机化合物更多相关视频
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