分子伴侣GroEL作为Cronobacter sakazakii的潜在毒性因子的表征
Dongdong Zhu1, Yufei Fan1, Xiaoyi Wang1
1State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Foods (Basel, Switzerland)
|September 28, 2023
概括
来自*C. sakazakii*的分子伴侣GroEL是一种增强细菌粘附和入侵的毒性因子. 抑制其基因可以降低细菌的毒性,并影响生理活动.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 食品安全 食品安全
背景情况:
- *C. sakazakii*中的分子伴侣GroEL对于适应热和干燥条件至关重要.
- 以前的研究表明,GroEL存在于细菌膜部分,并在引起免疫反应方面发挥作用.
- GroEL有助于*C. sakazakii*对食品安全和人类健康的威胁.
研究的目的:
- 为了阐明GroEL在*C. sakazakii*中的亚细胞局部.
- 研究 *C. sakazakii* GroEL. 的毒性作用和分泌机制.
- 确定GroEL在细菌粘附,入侵和其他生理过程中的作用.
主要方法:
- 在可溶和不可溶的细胞分数中对GroEL局部化的分析.
- 非减小的西方免疫洗以确定出口的GroEL的形式.
- 间接免疫光测定以评估重组GroEL对HCT-8细胞的附着性.
- NF-κB信号通路的激活和促炎性细胞因子的分析.
- 对紧结蛋白表达的评估.
- 基因沉默*grol*以评估对细菌粘附,入侵,生长,生物膜形成,运动性和形态学的影响.
主要成果:
- GroEL存在于活 *C. sakazakii* 细胞的可溶和不可溶的部分中.
- 出口的GroEL主要是单体形式,可以位于细菌表面.
- 再组合GroEL粘附于HCT-8细胞,激活NF-κB通路,增加促炎细胞因子,并降低紧结蛋白的调节,导致剂量依赖的毒性.
- 抑制 *grol* 基因表达显著减少了细菌的粘附和入侵.
- 缺少GroEL会导致生长受损,生物膜形成减少,机动性缺陷和细菌线异常.
结论:
- *C. sakazakii* GroEL是一种重要的毒性因子,对细菌生理活动至关重要.
- 分泌的GroEL增强了细菌的粘附和入侵能力.
- GroEL在细菌毒性和生存中发挥着多方面的作用.
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