从绿咖啡中提取的ACE抑制性的发现,使用in-silico和in-vitro方法
Haopeng Dai1,2, Min He1,2, Guilin Hu1,2
1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Foods (Basel, Switzerland)
|September 28, 2023
概括
绿色咖啡豆通过抑制血管酶-I转化酶 (ACE) 显示出作为抗高血压剂的潜力. 两种已识别的,IIPNEVY和ITPPVMLPP,在体外显示出显著的ACE抑制.
科学领域:
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
- 食品科学 食品科学 食品科学
背景情况:
- 高血压治疗通常涉及抑制血管素-I转化酶 (ACE),这是氨酸-血管素系统的关键调节剂.
- 来自食物蛋白质的生物活性因其健康益处而越来越多地被探索,包括抗高血压特性.
研究的目的:
- 为了研究来自绿色咖啡豆的生物活性的血管素-I转化酶 (ACE) 抑制作用.
- 使用in silico和in vitro方法识别和表征新的ACE抑制性.
主要方法:
- 绿色咖啡豆蛋白被用酸盐和热解来化.
- 通过LC-MS/MS和in silico选确定了生物活性.
- 进行了体外ACE抑制试验,分子对接和动力分析 (Lineweaver-Burk图).
主要成果:
- 两种新型,IIPNEVY (IC50 = 57.54 μM) 和ITPPVMLPP (IC50 = 40.37 μM),表现出显著的 ACE 抑制活性.
- 分子对接揭示了在ACE S1活性口袋附近的稳定酶复合体.
- 动力学研究表明,IIPNEVY作为非竞争性抑制剂,ITPPVMLPP作为混合型抑制剂.
结论:
- 绿色咖啡豆衍生具有强大的ACE抑制活性.
- 这些,特别是IIPNEVY和ITPPVMLPP,显示出作为天然抗高血压剂的前景.
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